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Literature summary extracted from

  • Bui, N.K.; Turk, S.; Buckenmaier, S.; Stevenson-Jones, F.; Zeuch, B.; Gobec, S.; Vollmer, W.
    Development of screening assays and discovery of initial inhibitors of pneumococcal peptidoglycan deacetylase PgdA (2011), Biochem. Pharmacol., 82, 43-52.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.104 2-[[4-(diethylamino)-2-hydroxyphenyl]carbonyl]benzoic acid 1 mM, 7% residual activity Streptococcus pneumoniae
3.5.1.104 N-(3-acetylphenyl)-2-[2-(hydrazinylcarbonothioyl)hydrazinyl]-2-(1-oxido-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)acetamide 1 mM, 29% residual activity Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.104 2.27
-
 4-yethylumbelliferyl acetate 37°C, pH 7.0 Streptococcus pneumoniae
3.5.1.104 2.38
-
 4-yethylumbelliferyl acetate 37°C, pH 8.0 Streptococcus pneumoniae
3.5.1.104 16.7
-
 4-nitrophenyl acetate 37°C, pH 7.0 Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.104 Streptococcus pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.104 4-methylumbelliferyl acetate + H2O
-
 Streptococcus pneumoniae 4-methylumbelliferol + acetate
-
 ?
3.5.1.104 4-nitrophenyl acetate + H2O
-
 Streptococcus pneumoniae 4-nitrophenol + acetate
-
 ?
3.5.1.104 acetylated peptidoglycan + H2O
-
 Streptococcus pneumoniae deacetylated peptidoglycan + acetate
-
 ?
3.5.1.104 additional information enzyme is inactive against peptidoglycans from Staphylococcus aureus, Staphylococcus carnosus or Escherichia coli. No substrate: N-acetylglucosamine Streptococcus pneumoniae ?
-
 ?
3.5.1.104 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Streptococcus pneumoniae N,N',N''-triacetylchitopentaose + N,N'-diacetylchitopentaose + N-acetylchitopentaose + acetate almost quantitative conversion to mono-, di- and tri-de-acetylated products ?
3.5.1.104 peptidoglycan from Streptococcus suis + H2O
-
 Streptococcus pneumoniae deacetylated peptidoglycan + acetate
-
 ?
3.5.1.104 peptidoglycan from wild-type Streptococcus pneumoniae + H2O
-
 Streptococcus pneumoniae deacetylated peptidoglycan + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.104 pgdA
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.104 0.28
-
 4-yethylumbelliferyl acetate 37°C, pH 7.0 Streptococcus pneumoniae
3.5.1.104 0.46
-
 4-nitrophenyl acetate 37°C, pH 7.0 Streptococcus pneumoniae
3.5.1.104 1.64
-
 4-yethylumbelliferyl acetate 37°C, pH 8.0 Streptococcus pneumoniae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.104 0.13
-
 37°C, pH 7.0 Streptococcus pneumoniae 2-[[4-(diethylamino)-2-hydroxyphenyl]carbonyl]benzoic acid
3.5.1.104 0.584
-
 37°C, pH 7.0 Streptococcus pneumoniae N-(3-acetylphenyl)-2-[2-(hydrazinylcarbonothioyl)hydrazinyl]-2-(1-oxido-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)acetamide

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.104 0.0288
-
 4-nitrophenyl acetate 37°C, pH 7.0 Streptococcus pneumoniae
3.5.1.104 0.129
-
 4-yethylumbelliferyl acetate 37°C, pH 7.0 Streptococcus pneumoniae
3.5.1.104 0.71
-
 4-yethylumbelliferyl acetate 37°C, pH 8.0 Streptococcus pneumoniae