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Literature summary extracted from

  • Riegler, H.; Herter, T.; Grishkovskaya, I.; Lude, A.; Ryngajllo, M.; Bolger, M.E.; Essigmann, B.; Usadel, B.
    Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana (2012), Biochem. J., 443, 427-437.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.4 expression in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.4 to 1.5 A resolution. The centre of the protein is a five-stranded beta-sheet, of which four strands are antiparallel-oriented Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.4 0.033
-
 acetyl-CoA pH 7.5, 30°C Arabidopsis thaliana
2.3.1.4 0.231
-
 D-glucosamine 6-phosphate pH 7.5, 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.4 membrane endomembrane system Arabidopsis thaliana 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.4 19200
-
 2 * 19200, calculated, 2 * 20000, SDS-PAGE Arabidopsis thaliana
2.3.1.4 20000
-
 2 * 19200, calculated, 2 * 20000, SDS-PAGE Arabidopsis thaliana
2.3.1.4 40300
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.4 Arabidopsis thaliana Q9LFU9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.4 additional information ubiquitously expressed in all organs examined Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.4 acetyl-CoA + D-glucosamine 6-phosphate
-
 Arabidopsis thaliana CoA + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.4 dimer 2 * 19200, calculated, 2 * 20000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.1.4 GNA
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.4 30 40
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.4 7 8
-
 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.4 0.52
-
 D-glucosamine 6-phosphate pH 7.5, 30°C Arabidopsis thaliana
2.3.1.4 0.55
-
 acetyl-CoA pH 7.5, 30°C Arabidopsis thaliana