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Literature summary extracted from

  • Shaw, D.J.; Dodgson, K.S.; White, G.F.
    Substrate specificity and other properties of the inducible S3 secondary alkylsulphohydrolase purified from the detergent-degrading bacterium Pseudomonas C12B (1980), Biochem. J., 187, 181-190.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.19 decan-5-yl sulfate
-
 Pseudomonas sp.
3.1.6.19 K+
-
 Pseudomonas sp.
3.1.6.19 octan-5-yl sulfate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.19 1.5
-
 (5R)-nonan-5-yl sulfate pH 7.5, 30°C Pseudomonas sp.
3.1.6.19 15
-
 (4R)-heptan-4-yl sulfate pH 7.5, 30°C Pseudomonas sp.
3.1.6.19 40
-
 (3R)-pentan-3-yl sulfate pH 7.5, 30°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.19 40000
-
 gel filtration Pseudomonas sp.
3.1.6.19 46000
-
 polyacrylamide gel under non-denaturing conditions Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.19 Pseudomonas sp.
-
-
-
3.1.6.19 Pseudomonas sp. C12B
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.19
-
 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.6.19 124.3
-
 pH 7.5, 30°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.19 (3R)-pentan-3-yl sulfate + H2O low activity Pseudomonas sp. pentan-3-ol + sulfate
-
 ?
3.1.6.19 (3R)-pentan-3-yl sulfate + H2O low activity Pseudomonas sp. C12B pentan-3-ol + sulfate
-
 ?
3.1.6.19 (4R)-heptan-4-yl sulfate + H2O the L-isomer is much more readily hydrolysed than the D-isomer. Enzymic hydrolysis of this group is accompanied by complete inversion of configuration at the asymmetric carbon atom Pseudomonas sp. (4S)-heptan-4-ol + sulfate
-
 ?
3.1.6.19 (4R)-heptan-4-yl sulfate + H2O the L-isomer is much more readily hydrolysed than the D-isomer. Enzymic hydrolysis of this group is accompanied by complete inversion of configuration at the asymmetric carbon atom Pseudomonas sp. C12B (4S)-heptan-4-ol + sulfate
-
 ?
3.1.6.19 (5R)-nonan-5-yl sulfate + H2O the L-isomer is much more readily hydrolysed than the D-isomer. Enzymic hydrolysis of this group is accompanied by complete inversion of configuration at the asymmetric carbon atom Pseudomonas sp. nonan-5-ol + sulfate
-
 ?
3.1.6.19 (5R)-nonan-5-yl sulfate + H2O the L-isomer is much more readily hydrolysed than the D-isomer. Enzymic hydrolysis of this group is accompanied by complete inversion of configuration at the asymmetric carbon atom Pseudomonas sp. C12B nonan-5-ol + sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.19 S3 secondary alkylsulphohydrolase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.6.19 30
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.19 7.5
-
 assay at Pseudomonas sp.
3.1.6.19 8.2
-
 hydrolysis of heptan-4-yl sulfate and nonan-5-yl sulfate Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.6.19 5 9.2 5.0: about of 40% of maximal activity with nonan-5-yl sulfate, about 80% of maximal activity with heptan-4-yl sulfate. pH 9.2: about 55% of maximal activity with nonan-5-yl sulfate Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.6.19 2.4
-
 decan-5-yl sulfate pH 7.5, 30°C Pseudomonas sp.
3.1.6.19 43
-
 octan-5-yl sulfate pH 7.5, 30°C Pseudomonas sp.