Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Marcondes, M.; Torquato, R.; Assis, D.; Juliano, M.; Hayashi, M.; Oliveira, V.
    Mitochondrial intermediate peptidase: Expression in Escherichia coli and improvement of its enzymatic activity detection with FRET substrates (2010), Biochem. Biophys. Res. Commun., 391, 123-128.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.59 DTT activates enzymatic activity up to 0.1 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.59 expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.59 1,10-phenanthroline total inhibition at 10 mM Homo sapiens
3.4.24.59 bestatin significant inhibition Homo sapiens
3.4.24.59 captopril
-
 Homo sapiens
3.4.24.59 DTT inhibits enzymatic activity above to 0.1 mM Homo sapiens
3.4.24.59 EDTA 90% inhibitiond by EDTA (10 mM) Homo sapiens
3.4.24.59 JA-2
-
 Homo sapiens
3.4.24.59 thiorphan
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.59 0.001
-
 Abz-GFSPFRQ-EDDnp pH 7.4, 37°C, 0.1 mM DTT Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.59 78640
-
 mass spectroscopy Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.59 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.59 affinity chromatography using Ni-Sepharose resin and ion exchange chromatography with a Hi-trap resource Q column Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.59 Abz-GFSPFRQ-EDDnp + H2O
-
 Homo sapiens Abz-GF + SPFRQ-EDDnp
-
 ?
3.4.24.59 Abz-RPPGFSPFRQ-EDDnp + H2O
-
 Homo sapiens Abz-RPPGF + SPFRQ-EDDnp
-
 ?
3.4.24.59 bradykinin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.59 hMIP
-
 Homo sapiens
3.4.24.59 mitochondrial intermediate peptidase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.59 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.59 7.4
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.59 0.0015
-
 thiorphan pH 7.4, 37°C Homo sapiens
3.4.24.59 0.007
-
 JA-2 pH 7.4, 37°C Homo sapiens
3.4.24.59 0.057
-
 captopril pH 7.4, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.24.59 40
-
 Abz-GFSPFRQ-EDDnp pH 7.4, 37°C, 0.1 mM DTT Homo sapiens