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Literature summary extracted from

  • Mayer, J.; Denger, K.; Hollemeyer, K.; Schleheck, D.; Cook, A.M.
    (R)-Cysteate-nitrogen assimilation by Cupriavidus necator H16 with excretion of 3-sulfolactate: a patchwork pathway (2012), Arch. Microbiol., 194, 949-957.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.15 66000
-
 x * 66000, SDS-PAGE Cupriavidus necator
2.6.1.1 43000
-
 SDS-PAGE Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.15 Cupriavidus necator
-
-
-
2.3.3.15 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
2.6.1.1 Cupriavidus necator
-
-
-
2.6.1.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 by anion-exchange chromatography Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
2.6.1.1 enriched enzyme is stable and can be stored at 4°C or -20°C for many weeks Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.15 2-sulfoacetaldehyde + phosphate
-
 Cupriavidus necator acetyl phosphate + sulfite
-
 ?
2.3.3.15 2-sulfoacetaldehyde + phosphate
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 acetyl phosphate + sulfite
-
 ?
2.6.1.1 3-aminopropanesulfonate + 2-oxoglutarate 3-aminopropanesulfonate = homotaurine, lower activity compared to L-cysteate Cupriavidus necator ?
-
 ?
2.6.1.1 3-aminopropanesulfonate + 2-oxoglutarate 3-aminopropanesulfonate = homotaurine, lower activity compared to L-cysteate Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
2.6.1.1 L-cysteate + 2-oxoglutarate L-cysteate = (R)-cysteate Cupriavidus necator 3-sulfopyruvate + L-glutamate
-
 ?
2.6.1.1 L-cysteate + 2-oxoglutarate L-cysteate = (R)-cysteate Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 3-sulfopyruvate + L-glutamate
-
 ?
2.6.1.1 L-cysteate + oxaloacetate
-
 Cupriavidus necator ?
-
 ?
2.6.1.1 L-cysteate + oxaloacetate
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
2.6.1.1 L-cysteate + pyruvate
-
 Cupriavidus necator ?
-
 ?
2.6.1.1 L-cysteate + pyruvate
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.15 ? x * 66000, SDS-PAGE Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
2.3.3.15 Xsc
-
 Cupriavidus necator
2.6.1.1 aoa/coa
-
 Cupriavidus necator
2.6.1.1 aspartate/(R)-cysteate:2-oxoglutarate aminotransferase
-
 Cupriavidus necator

Expression

EC Number Organism Comment Expression
2.3.3.15 Cupriavidus necator the enzyme is expressed inducibly during growth with cysteate as a nitrogen source up