EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.15 | gene otsA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B21(DE3) | Arthrobacter sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.15 | additional information | construction of N-loop exchange mutations of the otsA gene, the mutants show altered catalytic efficiencies at temperatures from 16 to 37°C compared to the wild-type enzyme, overview | Arthrobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.15 | additional information | - |
additional information | the high catalytic efficiency of OtsA results from the high affinity of the enzyme for uridine 5'-diphosphoglucose at low temperatures | Arthrobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | Arthrobacter sp. | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | Arthrobacter sp. Cjts | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.15 | Arthrobacter sp. | Q1KLW0 | gene otsA | - |
2.4.1.15 | Arthrobacter sp. Cjts | Q1KLW0 | gene otsA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.15 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain B21(DE3) by nickel affinity chromatography | Arthrobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Arthrobacter sp. | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Arthrobacter sp. Cjts | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.15 | OtsA | - |
Arthrobacter sp. |
2.4.1.15 | trehalose-6-phosphate synthase | - |
Arthrobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.15 | 37 | - |
assay at | Arthrobacter sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.15 | 16 | 37 | tested activity range | Arthrobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.15 | 7.5 | - |
assay at | Arthrobacter sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.15 | physiological function | the multifunctional protein, OtsA, is indispensable for bacterial survival in various environments. The catalytic efficiency of OtsA is affected by its N-loop at low temperatures. The flexibility of the OtsA N-loop is related to the growth temperature of the respective bacterial species | Arthrobacter sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.15 | additional information | - |
additional information | the high catalytic efficiency of OtsA results from the high affinity of the enzyme for uridine 5'-diphosphoglucose at low temperatures | Arthrobacter sp. |