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Literature summary extracted from

  • Hwang, S.K.; Nishi, A.; Satoh, H.; Okita, T.W.
    Rice endosperm-specific plastidial alpha-glucan phosphorylase is important for synthesis of short-chain malto-oligosaccharides (2010), Arch. Biochem. Biophys., 495, 82-92.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 glucose 1-phosphate production of a broad spectrum of MOSs (G4-G19) is stimulated both by phosphate and alpha-D-glucose 1-phosphate Oryza sativa
2.4.1.1 phosphate production of a broad spectrum of MOSs (G4-G19) is stimulated both by phosphate and alpha-D-glucose 1-phosphate Oryza sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.1 expression of His6-tagged Pho1 in Eschericia coli EA3457 cells Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 ADP-glucose 2fold inhibition at 4 mM Oryza sativa
2.4.1.1 Al3+
-
 Oryza sativa
2.4.1.1 ATP slight inhibition Oryza sativa
2.4.1.1 Co2+
-
 Oryza sativa
2.4.1.1 CTP slight inhibition Oryza sativa
2.4.1.1 Cu2+
-
 Oryza sativa
2.4.1.1 Fe2+
-
 Oryza sativa
2.4.1.1 GTP slight inhibition Oryza sativa
2.4.1.1 Hg2+
-
 Oryza sativa
2.4.1.1 phosphate Pho1 activity is strongly competitively inhibited by product phosphate in the synthesis reaction when amylopectin is the primer substrate, but this inhibition is less pronounced when short alpha-glucan chains are used as primers Oryza sativa
2.4.1.1 phosphoenol pyruvate slight inhibition Oryza sativa
2.4.1.1 Sn2+
-
 Oryza sativa
2.4.1.1 Zn2+
-
 Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 chloroplast Pho1 is plastidial Oryza sativa 9507
-
2.4.1.1 cytosol Pho2 Oryza sativa 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.1 Ca2+ stimulates Pho1 activity by 1.5-1.8fold Oryza sativa
2.4.1.1 Mg2+ stimulates Pho1 activity by 1.5-1.8fold Oryza sativa
2.4.1.1 Mn2+ stimulates Pho1 activity by 1.5-1.8fold Oryza sativa
2.4.1.1 additional information Na+, K+, NH4-, and Li+ have essentially no effect on Pho1 activity, and no effect by 4 mM DTT Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.1 additional information Oryza sativa Pho1 not only degrades maltohexaose but also extends them to synthesize longer malto-oligosaccharides, production of a broad spectrum of MOSs (G4-G19) is stimulated both by phosphate and alpha-D-glucose 1-phosphate ?
-
 ?
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate Oryza sativa
-
 [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Oryza sativa Q9ATK9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 seed Pho1 is endosperm-specific Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 additional information Pho1 not only degrades maltohexaose but also extends them to synthesize longer malto-oligosaccharides, production of a broad spectrum of MOSs (G4-G19) is stimulated both by phosphate and alpha-D-glucose 1-phosphate Oryza sativa ?
-
 ?
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Oryza sativa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate usage of four different assay methods Oryza sativa [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.1 alpha-glucan phosphorylase
-
 Oryza sativa
2.4.1.1 Pho1
-
 Oryza sativa
2.4.1.1 type L alpha-glucan phosphorylase
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 37
-
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6.5 7
-
 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 6 7.5 sharp decline in activity below pH 6.0 and above pH 7.5 Oryza sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 0.69
-
 phosphate Pho1 with amylopectin as primer in the reaction, pH 6.5, 22°C Oryza sativa
2.4.1.1 14.2
-
 phosphate Pho1 with short alpha-glucan chain as primer in the reaction, pH 6.5, 22°C Oryza sativa

General Information

EC Number General Information Comment Organism
2.4.1.1 evolution plants contain two major Pho forms: the plastidial Pho1 or PhoL (low glycogen affinity) encoded by the PHO1 gene and a cytosolic Pho2 or PhoH (high glycogen affinity) encoded by the PHO2 gene Oryza sativa
2.4.1.1 physiological function the endosperm-specific plastidial alpha-glucan phosphorylase is important for synthesis of short-chain malto-oligosaccharides, it has an essential role during the initiation process of starch biosynthesis during rice seed development Oryza sativa