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Literature summary extracted from

  • You, Z.; Omura, S.; Ikeda, H.; Cane, D.E.
    Pentalenolactone biosynthesis: Molecular cloning and assignment of biochemical function to PtlF, a short-chain dehydrogenase from Streptomyces avermitilis, and identification of a new biosynthetic intermediate (2007), Arch. Biochem. Biophys., 459, 233-240.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.340 expression in Escherichia coli Streptomyces avermitilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.340 0.0065
-
 1-deoxy-11beta-hydroxypentalenic acid pH 8.0, 22°C Streptomyces avermitilis
1.1.1.340 0.025
-
 NAD+ pH 8.0, 22°C Streptomyces avermitilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.340 30183
-
 x * 30183, MALDI-TOF, recombinant protein including His-tag Streptomyces avermitilis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.340 Streptomyces avermitilis Q82IY9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.340 1-deoxy-11beta-hydroxypentalenic acid + NAD+
-
 Streptomyces avermitilis 1-deoxy-11-oxopentalenic acid + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.340 ? x * 30183, MALDI-TOF, recombinant protein including His-tag Streptomyces avermitilis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.340 ptlF
-
 Streptomyces avermitilis
1.1.1.340 SAV_2933 locus name Streptomyces avermitilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.340 0.65
-
 1-deoxy-11beta-hydroxypentalenic acid pH 8.0, 22°C Streptomyces avermitilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.340 8
-
 and second maximum at pH 11.3 Streptomyces avermitilis
1.1.1.340 11.3
-
 activity increases from pH 9.0 to pH 11.3, and is 12fold higher at pH 11.3 than at pH 8.0 Streptomyces avermitilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.340 NAD+ 150fold preference for NAD+ over NADP+ Streptomyces avermitilis
1.1.1.340 NADP+ 150fold preference for NAD+ over NADP+ Streptomyces avermitilis