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Literature summary extracted from

  • Okada, S.; Devarenne, T.P.; Murakami, M.; Abe, H.; Chappell, J.
    Characterization of botryococcene synthase enzyme activity, a squalene synthase-like activity from the green microalga Botryococcus braunii, Race B (2004), Arch. Biochem. Biophys., 422, 110-118.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.96 Tween 80 increasing concentrations of Tween 80 up to 2% enhance enzyme activity approximately 2.5fold Botryococcus braunii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.97 expressed in Escherichia coli BL21(DE3) cells Botryococcus braunii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.96 squalestatin
-
 Botryococcus braunii
1.3.1.97 squalestatin
-
 Botryococcus braunii
1.3.1.97 Triton X-100
-
 Botryococcus braunii
1.3.1.97 Tween 80 enzyme activity is completely abolished by 0.5% (v/v) Tween 80 Botryococcus braunii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.96 membrane
-
 Botryococcus braunii 16020
-
1.3.1.97 membrane
-
 Botryococcus braunii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.96 Co2+ required for activity Botryococcus braunii
1.3.1.96 Mg2+ required for activity Botryococcus braunii
1.3.1.96 Mn2+ required for activity Botryococcus braunii
1.3.1.97 Mg2+ required for activity Botryococcus braunii
1.3.1.97 additional information no enzyme activity is evident with Co2+ or Mn2+ as a cofactor Botryococcus braunii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.97 52500
-
 x * 52500, estimated from amino acid sequence and SDS-PAGE Botryococcus braunii

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.96 Botryococcus braunii
-
 race B
-
1.3.1.96 Botryococcus braunii Berkeley (Showa)
-
 race B
-
1.3.1.97 Botryococcus braunii
-
 race B
-
1.3.1.97 Botryococcus braunii Berkeley (Showa)
-
 race B
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.96 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii squalene + diphosphate + NADP+
-
 ?
1.3.1.96 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii Berkeley (Showa) squalene + diphosphate + NADP+
-
 ?
1.3.1.97 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii C30 botryococcene + NADP+ + diphosphate
-
 ?
1.3.1.97 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii Berkeley (Showa) C30 botryococcene + NADP+ + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.97 ? x * 52500, estimated from amino acid sequence and SDS-PAGE Botryococcus braunii

Synonyms

EC Number Synonyms Comment Organism
1.3.1.96 squalene synthase
-
 Botryococcus braunii
1.3.1.97 botryococcene synthase
-
 Botryococcus braunii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.96 37
-
-
 Botryococcus braunii
1.3.1.97 37
-
-
 Botryococcus braunii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.96 7.3
-
 in MOPS buffer Botryococcus braunii
1.3.1.97 7.3
-
 in MOPS buffer Botryococcus braunii

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.96 NADPH
-
 Botryococcus braunii
1.3.1.97 NADPH
-
 Botryococcus braunii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.96 0.000001
-
 squalestatin at pH 7.3 and 37°C Botryococcus braunii
1.3.1.97 0.000001
-
 squalestatin at pH 7.3 and 37°C Botryococcus braunii

Expression

EC Number Organism Comment Expression
1.3.1.97 Botryococcus braunii gene expression is preferential during rapid growth. Enzyme activity is initially low in the cells, but rises greater than 10fold to a maximum by day 3, and then decreases gradually to almost undetectable levels by the end of the culture period up