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Literature summary extracted from

  • Kandasamy, V.; Vaidyanathan, H.; Djurdjevic, I.; Jayamani, E.; Ramachandran, K.; Buckel, W.; Jayaraman, G.; Ramalingam, S.
    Engineering Escherichia coli with acrylate pathway genes for propionic acid synthesis and its impact on mixed-acid fermentation (2013), Appl. Microbiol. Biotechnol., 97, 1191-1200.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.95 expressed in Escherichia coli Rosetta-gami 2(DE3)pLysS (VK-23) cells Anaerotignum propionicum
4.2.1.54 expressed in Escherichia coli Rosetta-gami 2(DE3)pLysS cells Anaerotignum propionicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.54 (R)-lactoyl-CoA Anaerotignum propionicum
-
 acryloyl-CoA + H2O
-
 ?
4.2.1.54 (R)-lactoyl-CoA Anaerotignum propionicum DSM 1682
-
 acryloyl-CoA + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.95 Anaerotignum propionicum G3KIM6 alpha subunit
-
1.3.1.95 Anaerotignum propionicum DSM 1682 G3KIM6 alpha subunit
-
4.2.1.54 Anaerotignum propionicum
-
-
-
4.2.1.54 Anaerotignum propionicum DSM 1682
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.95 0.02
-
 recombinant enzyme, in 0.1 M Tris pH 7.0, temperature not specified in the publication Anaerotignum propionicum
4.2.1.54 0.031
-
 recombinant enzyme, pH and temperature not specified in the publication Anaerotignum propionicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.95 acryloyl-CoA + NADH + H+
-
 Anaerotignum propionicum propanoyl-CoA + NAD+
-
 ?
1.3.1.95 acryloyl-CoA + NADH + H+
-
 Anaerotignum propionicum DSM 1682 propanoyl-CoA + NAD+
-
 ?
4.2.1.54 (R)-lactoyl-CoA
-
 Anaerotignum propionicum acryloyl-CoA + H2O
-
 ?
4.2.1.54 (R)-lactoyl-CoA
-
 Anaerotignum propionicum DSM 1682 acryloyl-CoA + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.95 Acr
-
 Anaerotignum propionicum
1.3.1.95 acryloyl-CoA reductase
-
 Anaerotignum propionicum
4.2.1.54 LCD
-
 Anaerotignum propionicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.95 FAD
-
 Anaerotignum propionicum
1.3.1.95 NADH
-
 Anaerotignum propionicum