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Literature summary extracted from

  • Park, S.; Lee, B.; Park, K.; Chong, Y.; Yoon, M.; Jeon, S.; Kim, D.
    Facilitation of polymerase chain reaction with thermostable inorganic pyrophosphatase from hyperthermophilic archaeon Pyrococcus horikoshii (2010), Appl. Microbiol. Biotechnol., 85, 807-812.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 pLysS(DE3) Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Mg2+ required Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Pyrococcus horikoshii
-
 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Pyrococcus horikoshii DSM 12428
-
 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Pyrococcus horikoshii O59570
-
-
3.6.1.1 Pyrococcus horikoshii DSM 12428 O59570
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Pyrococcus horikoshii 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O development and evaluation of a phosphate-based colorimetric assay to measure progress of PCR using the PPase-coupled enzyme assay, overview Pyrococcus horikoshii 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Pyrococcus horikoshii DSM 12428 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O development and evaluation of a phosphate-based colorimetric assay to measure progress of PCR using the PPase-coupled enzyme assay, overview Pyrococcus horikoshii DSM 12428 2 phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 inorganic pyrophosphatase
-
 Pyrococcus horikoshii
3.6.1.1 PPase
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 37
-
 assay at Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.1 37 95 active at Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.1 70 95 recombinant PhPPase, robust catalytic activity Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 7.5
-
 assay at Pyrococcus horikoshii