Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Weber, S.S.; Polli, F.; Boer, R.; Bovenberg, R.A.; Driessen, A.J.
    Increased penicillin production in Penicillium chrysogenum production strains via balanced overexpression of isopenicillin N acyltransferase (2012), Appl. Environ. Microbiol., 78, 7107-7113.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.164 gene penDE, lack of correlation between the protein and transcript levels of the IAT enzyme, quantitative real-time PCR expression analysis and gene copy number determination, overexpression of penDE in Penicillium chrysogenum strains DS47274, DS47273, and DS17690, introduction of penDE gene without introns into these strains in a random manner, via the nonhomologous end-joining pathway. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V Penicillium chrysogenum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.164 additional information in high-yielding penicillin production strains IAT is the limiting factor and that this limitation can be alleviated by a balanced overproduction of this enzyme. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O Penicillium chrysogenum
-
 CoA + penicillin G + L-2-aminohexanedioate
-
 ?
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O Penicillium chrysogenum DS17690
-
 CoA + penicillin G + L-2-aminohexanedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.164 Penicillium chrysogenum
-
 gene penDE
-
2.3.1.164 Penicillium chrysogenum DS17690
-
 gene penDE
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.164 additional information the protein level of IAT already saturates at low cluster copy numbers, lack of correlation between the protein and transcript levels of the IAT enzyme Penicillium chrysogenum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.164 phenoxyacetyl-CoA + isopenicillin N + H2O
-
 Penicillium chrysogenum CoA + penicillin V + L-2-aminohexanedioate
-
 ?
2.3.1.164 phenoxyacetyl-CoA + isopenicillin N + H2O
-
 Penicillium chrysogenum DS17690 CoA + penicillin V + L-2-aminohexanedioate
-
 ?
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O
-
 Penicillium chrysogenum CoA + penicillin G + L-2-aminohexanedioate
-
 ?
2.3.1.164 phenylacetyl-CoA + isopenicillin N + H2O
-
 Penicillium chrysogenum DS17690 CoA + penicillin G + L-2-aminohexanedioate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.164 IAT
-
 Penicillium chrysogenum
2.3.1.164 isopenicillin N acyltransferase
-
 Penicillium chrysogenum

General Information

EC Number General Information Comment Organism
2.3.1.164 malfunction penicillin production in high-yielding strains can be further improved by the overexpression of IAT while at very high levels of IAT the precursor 6-aminopenicillic acid accumulates Penicillium chrysogenum
2.3.1.164 physiological function IAT catalyzes a limiting step in high-yielding penicillin production strains Penicillium chrysogenum