BRENDA - Enzyme Database

Increased penicillin production in Penicillium chrysogenum production strains via balanced overexpression of isopenicillin N acyltransferase

Weber, S.S.; Polli, F.; Boer, R.; Bovenberg, R.A.; Driessen, A.J.; Appl. Environ. Microbiol. 78, 7107-7113 (2012) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.3.1.164
gene penDE, lack of correlation between the protein and transcript levels of the IAT enzyme, quantitative real-time PCR expression analysis and gene copy number determination, overexpression of penDE in Penicillium chrysogenum strains DS47274, DS47273, and DS17690, introduction of penDE gene without introns into these strains in a random manner, via the nonhomologous end-joining pathway. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V
Penicillium chrysogenum
Engineering
EC Number
Protein Variants
Commentary
Organism
2.3.1.164
additional information
in high-yielding penicillin production strains IAT is the limiting factor and that this limitation can be alleviated by a balanced overproduction of this enzyme. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V
Penicillium chrysogenum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.164
phenylacetyl-CoA + isopenicillin N + H2O
Penicillium chrysogenum
-
CoA + penicillin G + L-2-aminohexanedioate
-
-
?
2.3.1.164
phenylacetyl-CoA + isopenicillin N + H2O
Penicillium chrysogenum DS17690
-
CoA + penicillin G + L-2-aminohexanedioate
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.164
Penicillium chrysogenum
-
gene penDE
-
2.3.1.164
Penicillium chrysogenum DS17690
-
gene penDE
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.164
additional information
the protein level of IAT already saturates at low cluster copy numbers, lack of correlation between the protein and transcript levels of the IAT enzyme
Penicillium chrysogenum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.164
phenoxyacetyl-CoA + isopenicillin N + H2O
-
718653
Penicillium chrysogenum
CoA + penicillin V + L-2-aminohexanedioate
-
-
-
?
2.3.1.164
phenoxyacetyl-CoA + isopenicillin N + H2O
-
718653
Penicillium chrysogenum DS17690
CoA + penicillin V + L-2-aminohexanedioate
-
-
-
?
2.3.1.164
phenylacetyl-CoA + isopenicillin N + H2O
-
718653
Penicillium chrysogenum
CoA + penicillin G + L-2-aminohexanedioate
-
-
-
?
2.3.1.164
phenylacetyl-CoA + isopenicillin N + H2O
-
718653
Penicillium chrysogenum DS17690
CoA + penicillin G + L-2-aminohexanedioate
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.3.1.164
IAT
-
Penicillium chrysogenum
2.3.1.164
isopenicillin N acyltransferase
-
Penicillium chrysogenum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.164
gene penDE, lack of correlation between the protein and transcript levels of the IAT enzyme, quantitative real-time PCR expression analysis and gene copy number determination, overexpression of penDE in Penicillium chrysogenum strains DS47274, DS47273, and DS17690, introduction of penDE gene without introns into these strains in a random manner, via the nonhomologous end-joining pathway. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V
Penicillium chrysogenum
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
2.3.1.164
additional information
in high-yielding penicillin production strains IAT is the limiting factor and that this limitation can be alleviated by a balanced overproduction of this enzyme. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V
Penicillium chrysogenum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.3.1.164
phenylacetyl-CoA + isopenicillin N + H2O
Penicillium chrysogenum
-
CoA + penicillin G + L-2-aminohexanedioate
-
-
?
2.3.1.164
phenylacetyl-CoA + isopenicillin N + H2O
Penicillium chrysogenum DS17690
-
CoA + penicillin G + L-2-aminohexanedioate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.164
additional information
the protein level of IAT already saturates at low cluster copy numbers, lack of correlation between the protein and transcript levels of the IAT enzyme
Penicillium chrysogenum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.164
phenoxyacetyl-CoA + isopenicillin N + H2O
-
718653
Penicillium chrysogenum
CoA + penicillin V + L-2-aminohexanedioate
-
-
-
?
2.3.1.164
phenoxyacetyl-CoA + isopenicillin N + H2O
-
718653
Penicillium chrysogenum DS17690
CoA + penicillin V + L-2-aminohexanedioate
-
-
-
?
2.3.1.164
phenylacetyl-CoA + isopenicillin N + H2O
-
718653
Penicillium chrysogenum
CoA + penicillin G + L-2-aminohexanedioate
-
-
-
?
2.3.1.164
phenylacetyl-CoA + isopenicillin N + H2O
-
718653
Penicillium chrysogenum DS17690
CoA + penicillin G + L-2-aminohexanedioate
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.3.1.164
malfunction
penicillin production in high-yielding strains can be further improved by the overexpression of IAT while at very high levels of IAT the precursor 6-aminopenicillic acid accumulates
Penicillium chrysogenum
2.3.1.164
physiological function
IAT catalyzes a limiting step in high-yielding penicillin production strains
Penicillium chrysogenum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.3.1.164
malfunction
penicillin production in high-yielding strains can be further improved by the overexpression of IAT while at very high levels of IAT the precursor 6-aminopenicillic acid accumulates
Penicillium chrysogenum
2.3.1.164
physiological function
IAT catalyzes a limiting step in high-yielding penicillin production strains
Penicillium chrysogenum