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Literature summary extracted from

  • Malla, S.; Koffas, M.A.; Kazlauskas, R.J.; Kim, B.G.
    Production of 7-O-methyl aromadendrin, a medicinally valuable flavonoid, in Escherichia coli (2012), Appl. Environ. Microbiol., 78, 684-694.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.B75 synthesis the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin, which is important in medicinal applications, via sakuranetin from 4-coumaric acid in Escherichia coli, overview Streptomyces avermitilis
2.1.1.232 synthesis the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin, which is important in medicinal applications, via sakuranetin from 4-coumaric acid in Escherichia coli, overview Streptomyces avermitilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.232 subcloning in Escherichia coli strain DH5alpha, recombinant expression in Arabidopsis thaliana Streptomyces avermitilis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.B75 additional information the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin via sakuranetin from 4-coumaric acid in Escherichia coli, overview. In order to increase the availability of malonyl-CoA, a critical precursor of 7-O-methyl aromadendrin, genes for the acyl-CoA carboxylase alpha- and beta-subunits (nfa9890 and nfa9940), biotin ligase (nfa9950), and acetyl-CoA synthetase (nfa3550) from Nocardia farcinica were also introduced Streptomyces avermitilis
2.1.1.232 additional information the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin via sakuranetin from 4-coumaric acid in Escherichia coli, overview Streptomyces avermitilis
2.1.1.232 additional information production of 7-O-methyl aromadendrin in Escherichia coli strain BL21(DE3) recombinantly expressing the genes of several origins encoding enzymes 4-coumarate–coenzyme A ligase from Petroselinum crispum, chalcone synthase from Petunia hybrida, and chalcone flavanone isomerase from Medicago sativa to produce (2S)-naringenin, and further genes encoding flavanone-3-hydroxylase from Arabidopsis thaliana and the 7-O-methyltransferase from Streptomyces avermitilis to transform (2S)-naringenin into 7-O-methyl aromadendrin via dihydrokaempferol and (2S)-sakuranetin, method and pathway, overview Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin Streptomyces avermitilis
-
 S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin Streptomyces avermitilis MA-4680
-
 S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
2.1.1.232 S-adenosyl-L-methionine + dihydrokaempferol Streptomyces avermitilis
-
 S-adenosyl-L-homocysteine + 7-O-methyl aromadendrin 7-O-methyl aromadendrin is an aglycone moiety of one of the important flavonoid-glycosides found in several plants with various medicinal applications ?
2.1.1.232 S-adenosyl-L-methionine + dihydrokaempferol Streptomyces avermitilis MA-4680
-
 S-adenosyl-L-homocysteine + 7-O-methyl aromadendrin 7-O-methyl aromadendrin is an aglycone moiety of one of the important flavonoid-glycosides found in several plants with various medicinal applications ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.B75 Streptomyces avermitilis Q79ZE8 7-O-methyltransferase; gene SaOMT or SAV_2382
-
2.1.1.232 Streptomyces avermitilis Q79ZE8
-
-
2.1.1.232 Streptomyces avermitilis Q79ZE8 7-O-methyltransferase; gene SaOMT or SAV_2382
-
2.1.1.232 Streptomyces avermitilis MA-4680 Q79ZE8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.B75 S-adenosyl-L-methionine + (2S)-naringenin
-
 Streptomyces avermitilis S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
2.1.1.B75 S-adenosyl-L-methionine + (2S)-naringenin
-
 Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
2.1.1.B75 S-adenosyl-L-methionine + dihydrokaempferol
-
 Streptomyces avermitilis S-adenosyl-L-homocysteine + 7-O-methylaromadendrin i.e. 7-O-methyl-dihydrokaempferol ?
2.1.1.B75 S-adenosyl-L-methionine + dihydrokaempferol
-
 Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + 7-O-methylaromadendrin i.e. 7-O-methyl-dihydrokaempferol ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin
-
 Streptomyces avermitilis S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin
-
 Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
2.1.1.232 S-adenosyl-L-methionine + dihydrokaempferol
-
 Streptomyces avermitilis S-adenosyl-L-homocysteine + 7-O-methyl aromadendrin 7-O-methyl aromadendrin is an aglycone moiety of one of the important flavonoid-glycosides found in several plants with various medicinal applications ?
2.1.1.232 S-adenosyl-L-methionine + dihydrokaempferol
-
 Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + 7-O-methyl aromadendrin 7-O-methyl aromadendrin is an aglycone moiety of one of the important flavonoid-glycosides found in several plants with various medicinal applications ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.B75 7-O-methyltransferase
-
 Streptomyces avermitilis
2.1.1.B75 SaOMT
-
 Streptomyces avermitilis
2.1.1.232 7-O-methyltransferase
-
 Streptomyces avermitilis
2.1.1.232 SaOMT
-
 Streptomyces avermitilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.B75 S-adenosyl-L-methionine
-
 Streptomyces avermitilis
2.1.1.232 S-adenosyl-L-methionine
-
 Streptomyces avermitilis

General Information

EC Number General Information Comment Organism
2.1.1.B75 metabolism 7-O-methyl aromadendrin biosynthesis pathway from initial 4-coumaric acid, overview Streptomyces avermitilis