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Literature summary extracted from

  • Barney, B.M.; Wahlen, B.D.; Garner, E.; Wei, J.; Seefeldt, L.C.
    Differences in substrate specificities of five bacterial wax ester synthases (2012), Appl. Environ. Microbiol., 78, 5734-5745.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.75 analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Marinobacter nauticus
2.3.1.75 analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Acinetobacter baylyi
2.3.1.75 analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Rhodococcus jostii
2.3.1.75 analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Psychrobacter cryohalolentis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.75 expression in Escherichia coli Marinobacter nauticus
2.3.1.75 expression in Escherichia coli Acinetobacter baylyi
2.3.1.75 expression in Escherichia coli Rhodococcus jostii
2.3.1.75 expression in Escherichia coli Psychrobacter cryohalolentis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.75 Acinetobacter baylyi
-
-
-
2.3.1.75 Marinobacter nauticus
-
-
-
2.3.1.75 Psychrobacter cryohalolentis
-
-
-
2.3.1.75 Psychrobacter cryohalolentis K5
-
-
-
2.3.1.75 Rhodococcus jostii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.75 0.08
-
 substrate hexadecanol, pH 7.0, 22°C Acinetobacter baylyi
2.3.1.75 0.08
-
 substrate hexadecanol, pH 7.0, 22°C Psychrobacter cryohalolentis
2.3.1.75 0.12
-
 substrate hexadecanol, pH 7.0, 22°C Rhodococcus jostii
2.3.1.75 0.24
-
 substrate hexadecanol, isoform Ma2, pH 7.0, 22°C Marinobacter nauticus
2.3.1.75 0.68
-
 substrate hexadecanol, isoform Ma1, pH 7.0, 22°C Marinobacter nauticus
2.3.1.75 0.83
-
 substrate dodecanol, pH 7.0, 22°C Psychrobacter cryohalolentis
2.3.1.75 0.84
-
 substrate dodecanol, pH 7.0, 22°C Acinetobacter baylyi
2.3.1.75 3.37
-
 substrate dodecanol, pH 7.0, 22°C Rhodococcus jostii
2.3.1.75 4.96
-
 substrate dodecanol, isoform Ma2, pH 7.0, 22°C Marinobacter nauticus
2.3.1.75 37.3
-
 substrate dodecanol, isoform Ma1, pH 7.0, 22°C Marinobacter nauticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.75 decanol + palmitoyl-CoA and undecanol, best substrates Rhodococcus jostii decanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA
-
 Rhodococcus jostii dodecanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis dodecanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA best substrate Acinetobacter baylyi dodecanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA and undecanol, best substrates for both isoforms Ma1 and Ma2 Marinobacter nauticus dodecanyl palmitate + CoA
-
 ?
2.3.1.75 dodecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis K5 dodecanyl palmitate + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Marinobacter nauticus hexadecanyl palmitate + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Acinetobacter baylyi hexadecanyl palmitate + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis hexadecanyl palmitate + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis K5 hexadecanyl palmitate + CoA
-
 ?
2.3.1.75 additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Marinobacter nauticus ?
-
 ?
2.3.1.75 additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Acinetobacter baylyi ?
-
 ?
2.3.1.75 additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Psychrobacter cryohalolentis ?
-
 ?
2.3.1.75 additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Psychrobacter cryohalolentis K5 ?
-
 ?
2.3.1.75 tetradecanol + palmitoyl-CoA
-
 Acinetobacter baylyi tetradecanyl palmitate + CoA
-
 ?
2.3.1.75 tetradecanol + palmitoyl-CoA best substrate Psychrobacter cryohalolentis tetradecanyl palmitate + CoA
-
 ?
2.3.1.75 tetradecanol + palmitoyl-CoA best substrate Psychrobacter cryohalolentis K5 tetradecanyl palmitate + CoA
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA
-
 Acinetobacter baylyi undecanyl palmitate + CoA
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA and decanol, best substrates Rhodococcus jostii undecanyl palmitate + CoA
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA and dodecanol, best substrates for both isoforms Ma1 and Ma2 Marinobacter nauticus undecanyl palmitate + CoA
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis undecanyl palmitate + CoASH
-
 ?
2.3.1.75 undecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis K5 undecanyl palmitate + CoASH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.75 Rha1
-
 Rhodococcus jostii