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Literature summary extracted from

  • Drienovska, I.; Chovancova, E.; Koudelakova, T.; Damborsky, J.; Chaloupkova, R.
    Biochemical characterization of a novel haloalkane dehalogenase from a cold-adapted bacterium (2012), Appl. Environ. Microbiol., 78, 4995-4998.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.5 additional information
-
 additional information steady-state kinetic analysis with substrates 1-bromobutane and 1,3-dibromopropane, positive cooperative substrate binding, overview Psychrobacter cryohalolentis
3.8.1.5 3.83
-
 1-bromobutane pH 8.7, 37°C Psychrobacter cryohalolentis
3.8.1.5 5.77
-
 1,3-dibromopropane pH 8.7, 37°C Psychrobacter cryohalolentis

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Psychrobacter cryohalolentis
-
-
-
3.8.1.5 Psychrobacter cryohalolentis K5
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 (1-bromomethyl)cyclohexane + H2O low activity Psychrobacter cryohalolentis ?
-
 ?
3.8.1.5 1,2-dibromopropane + H2O low activity Psychrobacter cryohalolentis 2-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,2-dibromopropane + H2O low activity Psychrobacter cryohalolentis K5 2-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,3-dibromopropane + H2O high activity Psychrobacter cryohalolentis 3-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,3-dibromopropane + H2O high activity Psychrobacter cryohalolentis K5 3-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,3-diiodopropane + H2O
-
 Psychrobacter cryohalolentis 3-iodo-1-propanol + iodide
-
 ?
3.8.1.5 1,5-dichloropentane + H2O low activity Psychrobacter cryohalolentis 5-chloro-1-pentanol + chloride
-
 ?
3.8.1.5 1-bromo-2-chloroethane + H2O low activity Psychrobacter cryohalolentis 2-chloroethanol + bromide
-
 ?
3.8.1.5 1-bromo-3-chloropropane + H2O
-
 Psychrobacter cryohalolentis ?
-
 ?
3.8.1.5 1-bromobutane + H2O high activity Psychrobacter cryohalolentis 1-butanol + bromide
-
 ?
3.8.1.5 1-bromohexane + H2O high activity Psychrobacter cryohalolentis 1-hexanol + bromide
-
 ?
3.8.1.5 1-chlorohexane + H2O low activity Psychrobacter cryohalolentis n-hexanol + chloride
-
 ?
3.8.1.5 1-chlorohexane + H2O low activity Psychrobacter cryohalolentis K5 n-hexanol + chloride
-
 ?
3.8.1.5 1-iodobutane + H2O
-
 Psychrobacter cryohalolentis n-butanol + iodide
-
 ?
3.8.1.5 1-iodobutane + H2O
-
 Psychrobacter cryohalolentis K5 n-butanol + iodide
-
 ?
3.8.1.5 1-iodohexane + H2O
-
 Psychrobacter cryohalolentis n-hexanol + iodide
-
 ?
3.8.1.5 1-iodopropane + H2O
-
 Psychrobacter cryohalolentis n-propanol + iodide
-
 ?
3.8.1.5 2-iodobutane + H2O
-
 Psychrobacter cryohalolentis 2-butanol + iodide
-
 ?
3.8.1.5 3-chloro-2-methylpropene + H2O low activity Psychrobacter cryohalolentis ?
-
 ?
3.8.1.5 4-bromobutyronitrile + H2O
-
 Psychrobacter cryohalolentis ?
-
 ?
3.8.1.5 additional information substrate specificity profiles of DpcA, DpcA prefers brominated or iodinated substrates over chlorinated ones, overview Psychrobacter cryohalolentis ?
-
 ?
3.8.1.5 additional information substrate specificity profiles of DpcA, DpcA prefers brominated or iodinated substrates over chlorinated ones, overview Psychrobacter cryohalolentis K5 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.5 More sequence-based screening and enzyme identification, sequence comparisons, folding and secondary structure of DpcA, overview Psychrobacter cryohalolentis

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 DpcA
-
 Psychrobacter cryohalolentis
3.8.1.5 HLD
-
 Psychrobacter cryohalolentis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.5 40 50 with substrate 1-bromobutane Psychrobacter cryohalolentis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.5 1.1
-
 1-bromobutane pH 8.7, 37°C Psychrobacter cryohalolentis
3.8.1.5 2.72
-
 1,3-dibromopropane pH 8.7, 37°C Psychrobacter cryohalolentis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.5 8.7
-
 with substrate 1-bromobutane Psychrobacter cryohalolentis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.5 6.4 9.6 DpcA loses all activity below pH 6.4 and above pH 9.6 Psychrobacter cryohalolentis

General Information

EC Number General Information Comment Organism
3.8.1.5 evolution DpcA is a member of the haloalkane dehalogenase family Psychrobacter cryohalolentis