Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bourguet, F.A.; Souza, B.E.; Hinz, A.K.; Coleman, M.A.; Jackson, P.J.
    Characterization of a novel lytic protein encoded by the Bacillus cereus E33L gene ampD as a Bacillus anthracis antimicrobial protein (2012), Appl. Environ. Microbiol., 78, 3025-3027.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.28 31130
-
 x * 31130, calculated from amino acid sequence Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.28 additional information Bacillus cereus the enzyme shows very high lytic activity against Bacillus anthracis strain Sterne and Bacillus thuringiensis strain 97-27 isolates. More than 90% of the Bacillus anthracis cells are lysed by 1 nM protein and 99% are lysed by 50 nM protein in 60 min. Exposure to 10 nM for 10 min results in significantly less than 1% survival ?
-
 ?
3.5.1.28 additional information Bacillus cereus E33L the enzyme shows very high lytic activity against Bacillus anthracis strain Sterne and Bacillus thuringiensis strain 97-27 isolates. More than 90% of the Bacillus anthracis cells are lysed by 1 nM protein and 99% are lysed by 50 nM protein in 60 min. Exposure to 10 nM for 10 min results in significantly less than 1% survival ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.28 Bacillus cereus Q63AE6
-
-
3.5.1.28 Bacillus cereus E33L Q63AE6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.28 Ni-nitrilotriacetate resin column chromatography Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.28 additional information the enzyme shows very high lytic activity against Bacillus anthracis strain Sterne and Bacillus thuringiensis strain 97-27 isolates. More than 90% of the Bacillus anthracis cells are lysed by 1 nM protein and 99% are lysed by 50 nM protein in 60 min. Exposure to 10 nM for 10 min results in significantly less than 1% survival Bacillus cereus ?
-
 ?
3.5.1.28 additional information the enzyme shows very high lytic activity against Bacillus anthracis strain Sterne and Bacillus thuringiensis strain 97-27 isolates. More than 90% of the Bacillus anthracis cells are lysed by 1 nM protein and 99% are lysed by 50 nM protein in 60 min. Exposure to 10 nM for 10 min results in significantly less than 1% survival Bacillus cereus E33L ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.28 ? x * 31130, calculated from amino acid sequence Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.28 AmpD
-
 Bacillus cereus