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Literature summary extracted from

  • Nawabi, P.; Bauer, S.; Kyrpides, N.; Lykidis, A.
    Engineering Escherichia coli for biodiesel production utilizing a bacterial fatty acid methyltransferase (2011), Appl. Environ. Microbiol., 77, 8052-8061.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.15 expressed in Escherichia coli BL21(DE3)(pLysS) Mycobacterium marinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.15 0.046
-
 Decanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.077
-
 octanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.099
-
 3-hydroxydecanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.103
-
 dodecanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.129
-
 3-hydroxydodecanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.196
-
 3-hydroxyoctanoic acid pH 7.8, 37°C Mycobacterium marinum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.15 Mycobacterium marinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.15 immobilized metal ion affinity chromatography (Ni2+) Mycobacterium marinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.15 3-hydroxydecanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl 3-hydroxydecanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 3-hydroxydodecanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl 3-hydroxydodecanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 3-hydroxyoctanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl 3-hydroxyoctanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 decanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl decanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 dodecanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl dodecanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 octanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl octanoate + S-adenosyl-L-homocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.15 fatty acid methyltransferase
-
 Mycobacterium marinum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.15 358
-
 3-hydroxyoctanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 489
-
 Decanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 510
-
 octanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 1165
-
 dodecanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 1346
-
 3-hydroxydodecanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 1600
-
 3-hydroxydecanoic acid pH 7.8, 37°C Mycobacterium marinum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.15 1820
-
 3-hydroxyoctanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 6600
-
 octanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 10400
-
 3-hydroxydodecanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 10600
-
 Decanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 11300
-
 dodecanoic acid pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 16100
-
 3-hydroxydecanoic acid pH 7.8, 37°C Mycobacterium marinum