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Literature summary extracted from

  • Lim, C.; Fowler, Z.; Hueller, T.; Schaffer, S.; Koffas, M.
    High-yield resveratrol production in engineered Escherichia coli (2011), Appl. Environ. Microbiol., 77, 3451-3460.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.95 gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Arachis hypogaea
2.3.1.95 gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Pinus densiflora
2.3.1.95 gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Vitis vinifera
2.3.1.95 gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Pinus strobus
2.3.1.95 gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Polygonum cuspidatum
2.3.1.95 gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Pinus massoniana
2.3.1.95 gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Psilotum nudum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.95 acetyl-CoA competitive inhibition Arachis hypogaea
2.3.1.95 acetyl-CoA
-
 Pinus massoniana
2.3.1.95 acetyl-CoA competitive inhibition Pinus strobus
2.3.1.95 acetyl-CoA competitive inhibition Vitis vinifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.95 0.00287
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus strobus
2.3.1.95 0.00443
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Arachis hypogaea
2.3.1.95 0.00856
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Vitis vinifera
2.3.1.95 0.0119
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus massoniana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA Arachis hypogaea
-
 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA Pinus densiflora
-
 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA Vitis vinifera
-
 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA Pinus strobus
-
 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA Polygonum cuspidatum
-
 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA Pinus massoniana
-
 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA Psilotum nudum
-
 4 CoA + trans-resveratrol + 4 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.95 Arachis hypogaea
-
 gene sts
-
2.3.1.95 Pinus densiflora
-
 gene sts
-
2.3.1.95 Pinus massoniana
-
 gene sts
-
2.3.1.95 Pinus strobus
-
 gene sts
-
2.3.1.95 Polygonum cuspidatum
-
 gene sts
-
2.3.1.95 Psilotum nudum
-
 gene sts
-
2.3.1.95 Vitis vinifera
-
 gene sts
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.95 recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Arachis hypogaea
2.3.1.95 recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Pinus densiflora
2.3.1.95 recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Vitis vinifera
2.3.1.95 recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Pinus strobus
2.3.1.95 recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Polygonum cuspidatum
2.3.1.95 recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Pinus massoniana
2.3.1.95 recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Psilotum nudum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Arachis hypogaea 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Pinus densiflora 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Vitis vinifera 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Pinus strobus 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Polygonum cuspidatum 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Pinus massoniana 4 CoA + trans-resveratrol + 4 CO2
-
 ?
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Psilotum nudum 4 CoA + trans-resveratrol + 4 CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.95 stilbene synthase
-
 Arachis hypogaea
2.3.1.95 stilbene synthase
-
 Pinus densiflora
2.3.1.95 stilbene synthase
-
 Vitis vinifera
2.3.1.95 stilbene synthase
-
 Pinus strobus
2.3.1.95 stilbene synthase
-
 Polygonum cuspidatum
2.3.1.95 stilbene synthase
-
 Pinus massoniana
2.3.1.95 stilbene synthase
-
 Psilotum nudum
2.3.1.95 STS
-
 Arachis hypogaea
2.3.1.95 STS
-
 Pinus densiflora
2.3.1.95 STS
-
 Vitis vinifera
2.3.1.95 STS
-
 Pinus strobus
2.3.1.95 STS
-
 Polygonum cuspidatum
2.3.1.95 STS
-
 Pinus massoniana
2.3.1.95 STS
-
 Psilotum nudum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.95 30
-
 assay at Arachis hypogaea
2.3.1.95 30
-
 assay at Pinus densiflora
2.3.1.95 30
-
 assay at Vitis vinifera
2.3.1.95 30
-
 assay at Pinus strobus
2.3.1.95 30
-
 assay at Polygonum cuspidatum
2.3.1.95 30
-
 assay at Pinus massoniana
2.3.1.95 30
-
 assay at Psilotum nudum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.95 0.0005
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus strobus
2.3.1.95 0.0017
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Arachis hypogaea
2.3.1.95 0.0023
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus massoniana
2.3.1.95 0.003
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.95 4
-
 assay at Arachis hypogaea
2.3.1.95 4
-
 assay at Pinus densiflora
2.3.1.95 4
-
 assay at Vitis vinifera
2.3.1.95 4
-
 assay at Pinus strobus
2.3.1.95 4
-
 assay at Polygonum cuspidatum
2.3.1.95 4
-
 assay at Pinus massoniana
2.3.1.95 4
-
 assay at Psilotum nudum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.95 0.02
-
 acetyl-CoA pH 4.0, 30°C, uncompetitive inhibition Vitis vinifera
2.3.1.95 0.04
-
 acetyl-CoA pH 4.0, 30°C, uncompetitive inhibition Pinus massoniana
2.3.1.95 0.07
-
 acetyl-CoA pH 4.0, 30°C, recombinant STS, uncompetitive inhibition Arachis hypogaea
2.3.1.95 0.15
-
 acetyl-CoA pH 4.0, 30°C, uncompetitive inhibition Pinus strobus
2.3.1.95 0.46
-
 acetyl-CoA pH 4.0, 30°C, competitive inhibition Vitis vinifera
2.3.1.95 0.52
-
 acetyl-CoA pH 4.0, 30°C, recombinant STS, competitive inhibition Arachis hypogaea
2.3.1.95 0.787
-
 acetyl-CoA pH 4.0, 30°C, competitive inhibition Pinus massoniana
2.3.1.95 0.97
-
 acetyl-CoA pH 4.0, 30°C, competitive inhibition Pinus strobus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.95 0.107
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus massoniana
2.3.1.95 0.16
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus strobus
2.3.1.95 0.21
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Vitis vinifera
2.3.1.95 0.314
-
 4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Arachis hypogaea