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Literature summary extracted from

  • Hasan, K.; Fortova, A.; Koudelakova, T.; Chaloupkova, R.; Ishitsuka, M.; Nagata, Y.; Damborsky, J.; Prokop, Z.
    Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58 (2011), Appl. Environ. Microbiol., 77, 1881-1884.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 gene datA, DNA and amnino acid sequence determination and analysis Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.5 additional information
-
 additional information DatA shows substrate-dependent and cooperative kinetics Agrobacterium tumefaciens
3.8.1.5 0.07
-
 1-Chlorohexane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 0.14
-
 1-bromohexane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 0.2
-
 1,3-diiodopropane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 0.63
-
 1-iodohexane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 2.15
-
 1,3-dibromopropane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 4
-
 1,3-Dichloropropane pH 8.6, 37°C, DatA Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Agrobacterium tumefaciens E2RV69 gene datA
-
3.8.1.5 Agrobacterium tumefaciens C58 / ATCC 33970 E2RV69 gene datA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1,3-dibromopropane + H2O
-
 Agrobacterium tumefaciens 3-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,3-dibromopropane + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 3-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,3-dichloropropane + H2O
-
 Agrobacterium tumefaciens 3-chloropropan-1-ol + chloride
-
 ?
3.8.1.5 1,3-diiodopropane + H2O
-
 Agrobacterium tumefaciens 3-iodo-1-propanol + iodide
-
 ?
3.8.1.5 1-bromohexane + H2O
-
 Agrobacterium tumefaciens n-hexanol + bromide
-
 ?
3.8.1.5 1-bromohexane + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 n-hexanol + bromide
-
 ?
3.8.1.5 1-chlorohexane + H2O
-
 Agrobacterium tumefaciens n-hexanol + chloride
-
 ?
3.8.1.5 1-chlorohexane + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 n-hexanol + chloride
-
 ?
3.8.1.5 1-iodohexane + H2O
-
 Agrobacterium tumefaciens n-hexanol + iodide
-
 ?
3.8.1.5 1-iodohexane + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 n-hexanol + iodide
-
 ?
3.8.1.5 2-bromobutane + H2O
-
 Agrobacterium tumefaciens 2-butanol + bromide
-
 ?
3.8.1.5 2-bromohexane + H2O
-
 Agrobacterium tumefaciens 2-hexanol + bromide
-
 ?
3.8.1.5 2-bromopentane + H2O
-
 Agrobacterium tumefaciens 2-pentanol + bromide
-
 ?
3.8.1.5 additional information DatA shows excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters, racemic beta-bromoalkanes and alpha-bromoesters, overview. No activity with methyl and ethyl 2-bromobutyrate Agrobacterium tumefaciens ?
-
 ?
3.8.1.5 additional information DatA shows excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters, racemic beta-bromoalkanes and alpha-bromoesters, overview. No activity with methyl and ethyl 2-bromobutyrate Agrobacterium tumefaciens C58 / ATCC 33970 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.5 dimer
-
 Agrobacterium tumefaciens

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 DatA
-
 Agrobacterium tumefaciens
3.8.1.5 HLD
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.5 22 37 assay at room temperature or 37°C Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.5 0.008
-
 1,3-Dichloropropane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 0.039
-
 1-Chlorohexane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 0.26
-
 1-iodohexane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 1.2
-
 1,3-diiodopropane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 1.47
-
 1,3-dibromopropane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 1.7
-
 1-bromohexane pH 8.6, 37°C, DatA Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.5 8.2 8.6 assay at Agrobacterium tumefaciens

General Information

EC Number General Information Comment Organism
3.8.1.5 additional information DatA possesses a unique pair of halide-stabilizing residues, Asn-Tyr, not reported in other known haloalkane dehalogenases Agrobacterium tumefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.8.1.5 0.002
-
 1,3-Dichloropropane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 0.42
-
 1-iodohexane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 0.58
-
 1-Chlorohexane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 0.69
-
 1,3-dibromopropane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 6
-
 1,3-diiodopropane pH 8.6, 37°C, DatA Agrobacterium tumefaciens
3.8.1.5 12.2
-
 1-bromohexane pH 8.6, 37°C, DatA Agrobacterium tumefaciens