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Literature summary extracted from

  • Bonnin, R.A.; Ocampo-Sosa, A.A.; Poirel, L.; Guet-Revillet, H.; Nordmann, P.
    Biochemical and genetic characterization of carbapenem-hydrolyzing beta-lactamase OXA-229 from Acinetobacter bereziniae (2012), Antimicrob. Agents Chemother., 56, 3923-3927.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expression in Escherichia coli Acinetobacter bereziniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.00003
-
 meropenem 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 0.029
-
 piperacillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 0.03
-
 Imipenem 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 0.127
-
 ampicillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 0.165
-
 cephalothin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 0.343
-
 ticarcillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 0.405
-
 amoxicillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 1.85
-
 Oxacillin 30°C, pH not specified in the publication Acinetobacter bereziniae

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Acinetobacter bereziniae I6S7G0
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 12.5
-
 substrate benzylpenicillin, 30°C, pH not specified in the publication Acinetobacter bereziniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 amoxicillin + H2O
-
 Acinetobacter bereziniae (2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Acinetobacter bereziniae (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Acinetobacter bereziniae (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Acinetobacter bereziniae (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Acinetobacter bereziniae (5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Acinetobacter bereziniae (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Acinetobacter bereziniae (2R,4S)-2-[(R)-carboxy[[(5-methyl-3-phenyl-1,2-oxazol-4-yl)carbonyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Acinetobacter bereziniae (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ticarcillin + H2O
-
 Acinetobacter bereziniae (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 OXA-229
-
 Acinetobacter bereziniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.01
-
 meropenem 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 0.09
-
 Imipenem 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 0.2
-
 cephalothin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 1
-
 ampicillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 1.7
-
 ticarcillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 2.2
-
 piperacillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 4.2
-
 Oxacillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 11
-
 benzylpenicillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 15.2
-
 amoxicillin 30°C, pH not specified in the publication Acinetobacter bereziniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.6 0.1
-
 meropenem 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 1
-
 cephalothin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 2
-
 Oxacillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 3
-
 Imipenem 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 5
-
 ticarcillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 8
-
 ampicillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 38
-
 amoxicillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 80
-
 piperacillin 30°C, pH not specified in the publication Acinetobacter bereziniae
3.5.2.6 150
-
 benzylpenicillin 30°C, pH not specified in the publication Acinetobacter bereziniae