Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gutierrez, B.; Escudero, J.A.; San Millan, A.; Hidalgo, L.; Carrilero, L.; Ovejero, C.M.; Santos-Lopez, A.; Thomas-Lopez, D.; Gonzalez-Zorn, B.
    Fitness cost and interference of Arm/Rmt aminoglycoside resistance with the RsmF housekeeping methyltransferases (2012), Antimicrob. Agents Chemother., 56, 2335-2341.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.178 gene rsmF, expression of wild-type enzyme in Escherichi coli Top10 cells Escherichia coli
2.1.1.179 gene rmtC, expression of wild-type enzyme in Escherichi coli Top10 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.178 additional information construction of a series of in-frame knockout and knock-in mutants of Escherichia coli, corresponding to the genotypes rsmF+, DELTArsmF, rsmF+ rmtC+, and DELTArsmF rmtC+, mutant strain growth kinetics, overview Escherichia coli
2.1.1.179 additional information construction of a series of in-frame knockout and knock-in mutants of Escherichia coli, corresponding to the genotypes rsmF+, DELTArsmF, rsmF+ rmtC+, and DELTArsmF rmtC+, mutant strain growth kinetics, overview Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.178 additional information RmtC, EC 2.1.1.179, in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA Escherichia coli nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA Escherichia coli BW25113 nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
2.1.1.179 additional information Escherichia coli RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview ?
-
 ?
2.1.1.179 additional information Escherichia coli BW25113 RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview ?
-
 ?
2.1.1.179 S-adenosyl-L-methionine + cytosine1405 in 16S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
 ?
2.1.1.179 S-adenosyl-L-methionine + cytosine1405 in 16S rRNA Escherichia coli BW25113
-
 S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.178 Escherichia coli
-
 i.e. strain BB1074, gene rsmF
-
2.1.1.178 Escherichia coli BW25113
-
 i.e. strain BB1074, gene rsmF
-
2.1.1.179 Escherichia coli
-
 i.e. strain BB1074, gene rmtC
-
2.1.1.179 Escherichia coli BW25113
-
 i.e. strain BB1074, gene rmtC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA Escherichia coli BW25113 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
-
 Escherichia coli BW25113 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
2.1.1.179 additional information RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview Escherichia coli ?
-
 ?
2.1.1.179 additional information RmtC in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview Escherichia coli BW25113 ?
-
 ?
2.1.1.179 S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
 ?
2.1.1.179 S-adenosyl-L-methionine + cytosine1405 in 16S rRNA
-
 Escherichia coli BW25113 S-adenosyl-L-homocysteine + 5-methylcytosine1405 in 16S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.178 housekeeping methyltransferase RsmF
-
 Escherichia coli
2.1.1.178 RsmF housekeeping methyltransferase
-
 Escherichia coli
2.1.1.179 methyltransferase RmtC
-
 Escherichia coli
2.1.1.179 RmtC
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.178 S-adenosyl-L-methionine
-
 Escherichia coli
2.1.1.179 S-adenosyl-L-methionine
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.178 malfunction DELTArsmF null mutant bacteria have a decreased susceptibility to aminoglycosides, including 4,6- and 4,5-deoxystreptamine aminoglycosides Escherichia coli
2.1.1.178 physiological function the housekeeping methylation at C1407 is involved in intrinsic aminoglycoside susceptibility in Escherichia coli Escherichia coli
2.1.1.179 additional information acquisition of rmtC does not entail a fitness cost for the bacterium Escherichia coli
2.1.1.179 physiological function the enzyme confers high-level resistance to 4,6-disubstituted aminoglycosides through methylation of the G1405 residue in the 16S rRNA. RmtC impedes methylation by the housekeeping methyltransferase RsmF, EC 2.1.1.178, at position C1407 Escherichia coli