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Literature summary extracted from

  • El Salabi, A.; Borra, P.S.; Toleman, M.A.; Samuelsen, O.; Walsh, T.R.
    Genetic and biochemical characterization of a novel metallo-beta-lactamase, TMB-1, from an Achromobacter xylosoxidans strain isolated in Tripoli, Libya (2012), Antimicrob. Agents Chemother., 56, 2241-2245.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.009
-
 cefuroxime pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.027
-
 ampicillin pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.031
-
 ceftazidime pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.031
-
 ertapenem pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.069
-
 cefoxitin pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.072
-
 piperacillin pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.075
-
 meropenem pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.2
-
 Imipenem pH 7.2, 25°C Achromobacter sp. sallbros1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Achromobacter sp. sallbros1 E7BCS7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Achromobacter sp. sallbros1 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 aztreonam + H2O
-
 Achromobacter sp. sallbros1 [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Achromobacter sp. sallbros1 (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Achromobacter sp. sallbros1 (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Achromobacter sp. sallbros1 (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ertapenem + H2O
-
 Achromobacter sp. sallbros1 (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([(3S,5S)-5-[(3-carboxyphenyl)carbamoyl]pyrrolidin-3-yl]sulfanyl)-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Achromobacter sp. sallbros1 (5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Achromobacter sp. sallbros1 (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Achromobacter sp. sallbros1 (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 class D beta lactamase
-
 Achromobacter sp. sallbros1
3.5.2.6 TMB-1
-
 Achromobacter sp. sallbros1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.01
-
 aztreonam or below, pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.07
-
 ceftazidime pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.1
-
 cefuroxime pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.3
-
 cefoxitin pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 0.4
-
 ertapenem pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 1.4
-
 meropenem pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 1.7
-
 Imipenem pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 3.3
-
 ampicillin pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 3.3
-
 piperacillin pH 7.2, 25°C Achromobacter sp. sallbros1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.6 2
-
 ceftazidime pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 4
-
 cefoxitin pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 9
-
 Imipenem pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 11
-
 cefuroxime pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 13
-
 ertapenem pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 19
-
 meropenem pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 46
-
 piperacillin pH 7.2, 25°C Achromobacter sp. sallbros1
3.5.2.6 122
-
 ampicillin pH 7.2, 25°C Achromobacter sp. sallbros1