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Literature summary extracted from

  • Borgianni, L.; Prandi, S.; Salden, L.; Santella, G.; Hanson, N.D.; Rossolini, G.M.; Docquier, J.D.
    Genetic context and biochemical characterization of the IMP-18 metallo-beta-lactamase identified in a Pseudomonas aeruginosa isolate from the United States (2011), Antimicrob. Agents Chemother., 55, 140-145.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0001
-
 cephalotin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.0008
-
 cefepime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.00084
-
 meropenem pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.0013
-
 ceftazidime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.0026
-
 ertapenem pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.003
-
 cefotaxime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.007
-
 Imipenem pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.007
-
 cefuroxime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.009
-
 nitrocefin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.011
-
 cefoxitin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.09
-
 benzylpenicillin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.103
-
 ampicillin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.18
-
 ticarcillin pH 7.5, 30°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Pseudomonas aeruginosa Q5U807
-
-
3.5.2.6 Pseudomonas aeruginosa PS297 Q5U807
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Pseudomonas aeruginosa PS297 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefepime + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefepime + H2O
-
 Pseudomonas aeruginosa PS297 (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Pseudomonas aeruginosa PS297 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Pseudomonas aeruginosa PS297 (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Pseudomonas aeruginosa PS297 (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalotin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ertapenem + H2O
-
 Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([(3S,5S)-5-[(3-carboxyphenyl)carbamoyl]pyrrolidin-3-yl]sulfanyl)-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Pseudomonas aeruginosa (5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ticarcillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 IMP-18
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.03
-
 ertapenem pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.05
-
 meropenem pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.35
-
 cefepime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.4
-
 cephalotin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.7
-
 cefotaxime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 0.9
-
 cefuroxime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 1
-
 ceftazidime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 2
-
 cefoxitin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 17
-
 Imipenem pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 30
-
 ticarcillin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 54
-
 benzylpenicillin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 55
-
 nitrocefin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 65
-
 ampicillin pH 7.5, 30°C Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.6 1.4
-
 meropenem pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 6
-
 ertapenem pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 130
-
 cefuroxime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 150
-
 ticarcillin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 180
-
 cefoxitin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 230
-
 cefotaxime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 440
-
 cefepime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 620
-
 benzylpenicillin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 630
-
 ampicillin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 770
-
 ceftazidime pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 2400
-
 Imipenem pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 4000
-
 cephalotin pH 7.5, 30°C Pseudomonas aeruginosa
3.5.2.6 6100
-
 nitrocefin pH 7.5, 30°C Pseudomonas aeruginosa