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Literature summary extracted from

  • Lassaux, P.; Traore, D.A.; Loisel, E.; Favier, A.; Docquier, J.D.; Sohier, J.S.; Laurent, C.; Bebrone, C.; Frere, J.M.; Ferrer, J.L.; Galleni, M.
    Biochemical and structural characterization of the subclass B1 metallo-beta-lactamase VIM-4 (2011), Antimicrob. Agents Chemother., 55, 1248-1255.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.6 dizinc form, to 1.9 A resolution Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.003
-
 Imipenem pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 0.008
-
 meropenem pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 0.011
-
 cefuroxime pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 0.014
-
 cephalotin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 0.022
-
 nitrocefin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 0.031
-
 carbenicillin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 0.066
-
 biapenem pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 0.18
-
 benzylpenicillin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 3
-
 ampicillin pH 7.0, 25°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zn2+ Zn2+ ions have a pronounced stabilizing effect and are necessary for preserving the structure Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Pseudomonas aeruginosa Q8KRJ3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 biapenem + H2O
-
 Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate
-
 ?
3.5.2.6 carbenicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalotin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Pseudomonas aeruginosa (5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 VIM-4
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.6 44.4
-
 and 56.6, transitions in presence of a 10:1 ratio of Zn2+ Pseudomonas aeruginosa
3.5.2.6 56.6
-
 and 44.4, transitions in presence of a 10:1 ratio of Zn2+ Pseudomonas aeruginosa
3.5.2.6 58.5
-
 melting temperature, native enzyme Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 7
-
 meropenem pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 15
-
 biapenem pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 70
-
 Imipenem pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 100
-
 carbenicillin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 120
-
 cefuroxime pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 210
-
 ampicillin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 500
-
 cephalotin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 650
-
 benzylpenicillin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 690
-
 nitrocefin pH 7.0, 25°C Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.6 71
-
 ampicillin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 230
-
 biapenem pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 875
-
 meropenem pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 3100
-
 benzylpenicillin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 3300
-
 carbenicillin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 6000
-
 cefuroxime pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 23000
-
 Imipenem pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 31000
-
 nitrocefin pH 7.0, 25°C Pseudomonas aeruginosa
3.5.2.6 36000
-
 cephalotin pH 7.0, 25°C Pseudomonas aeruginosa