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Literature summary extracted from
- A fluorescence-based assay for measuring N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase activity (2011), Anal. Biochem., 414, 278-281.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.103 | analysis | fluorescence-based assay for measuring N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase activity that does not require HPLC analysis and can be carried out in multiwell plates. The fluorescamine-based assay can be used to determine the steady-state parameters for the deacetylation of N-acetyl-glucosamine by MshB | Mycolicibacterium smegmatis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.103 | Mycolicibacterium smegmatis | - |
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Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.103 | 1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H2O | the inositol moiety primarily contributes to the affinity of N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside for MshB | Mycolicibacterium smegmatis | 1-(2-amino-2-deoxy-alpha-D-glucopyranoside)-1D-myo-inositol + acetate | - |
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