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Literature summary extracted from
- Lysine transporters in human trypanosomatid pathogens (2012), Amino Acids, 42, 347-360.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.4.2.1 | L-asparagine | slight activation | Trypanosoma cruzi |  |
| 7.4.2.1 | L-cysteine | slight activation | Leishmania donovani | |
| 7.4.2.1 | L-threonine | slight activation | Leishmania donovani | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.4.2.1 | gene LdAAP7, phylogenetic analysis, functional expression in enzyme-deficient Saccharomyces cerevisiae mutant strain 22DELTA7AA | Leishmania donovani |
| 7.4.2.1 | gene TcAAP7, phylogenetic analysis, functional expression in enzyme-deficient Saccharomyces cerevisiae mutant strain 22DELTA7AA. Functional overexpression of GFP-tagged TcAAP7 | Trypanosoma cruzi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.4.2.1 | glycine | - |
Trypanosoma cruzi | |
| 7.4.2.1 | L-aspartate | slight inhibition | Leishmania donovani | |
| 7.4.2.1 | L-cysteine | - |
Trypanosoma cruzi | |
| 7.4.2.1 | L-histidine | slight inhibition | Trypanosoma cruzi | |
| 7.4.2.1 | L-methionine | - |
Trypanosoma cruzi | |
| 7.4.2.1 | L-threonine | - |
Trypanosoma cruzi | |
| 7.4.2.1 | additional information | no or poor inhibition by L-alanine, L-asparagine, L-arginine, L-proline, L-serine, glycine, L-methionine, L-threonine, L-histidine, and L-cysteine | Leishmania donovani | |
| 7.4.2.1 | additional information | no inhibition by L-aspartate, L-arginine, L-asparagine, L-alanine, and L-serine | Trypanosoma cruzi | |
| 7.4.2.1 | proline | - |
Trypanosoma cruzi | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.4.2.1 | 0.00736 | - |
L-lysine/out | pH 4.5, 30°C, recombinant enzyme | Leishmania donovani | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.4.2.1 | cell surface | - |
Leishmania donovani | 9986 | - |
| 7.4.2.1 | flagellum | - |
Leishmania donovani | - |
- |
| 7.4.2.1 | membrane | - |
Leishmania donovani | 16020 | - |
| 7.4.2.1 | membrane | mainly to a membrane-bound structure or invagination close to the kinetoplast, the latter corresponding to the flagellar pocket or associated structures, such as the cytostome or contractile vacuole | Trypanosoma cruzi | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.4.2.1 | ATP + H2O + L-lysine/out | Leishmania donovani | - |
ADP + phosphate + L-lysine/in | - |
? | |
| 7.4.2.1 | ATP + H2O + L-lysine/out | Trypanosoma cruzi | - |
ADP + phosphate + L-lysine/in | - |
? | |
| 7.4.2.1 | ATP + H2O + polar amino acid/out | Leishmania donovani | - |
ADP + phosphate + polar amino acid/in | - |
? | |
| 7.4.2.1 | ATP + H2O + polar amino acid/out | Trypanosoma cruzi | - |
ADP + phosphate + polar amino acid/in | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.4.2.1 | Leishmania donovani | Q208R5 | gene LdAAP7 | - |
| 7.4.2.1 | Trypanosoma cruzi | Q4DMS3 | gene TcAAP7 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.4.2.1 | epimastigote | - |
Trypanosoma cruzi | - |
| 7.4.2.1 | promastigote | - |
Leishmania donovani | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.4.2.1 | ATP + H2O + L-lysine/out | - |
Leishmania donovani | ADP + phosphate + L-lysine/in | - |
? | |
| 7.4.2.1 | ATP + H2O + L-lysine/out | - |
Trypanosoma cruzi | ADP + phosphate + L-lysine/in | - |
? | |
| 7.4.2.1 | ATP + H2O + polar amino acid/out | - |
Leishmania donovani | ADP + phosphate + polar amino acid/in | - |
? | |
| 7.4.2.1 | ATP + H2O + polar amino acid/out | - |
Trypanosoma cruzi | ADP + phosphate + polar amino acid/in | - |
? | |
| 7.4.2.1 | additional information | specificity of L-lysine transport in wild-type cells and in recombinantly parasite AAP7 expressing yeast 22DELTA7AA cells, overview | Leishmania donovani | ? | - |
? | |
| 7.4.2.1 | additional information | specificity of L-lysine transport in wild-type cells, overview | Trypanosoma cruzi | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.4.2.1 | AAP7 | - |
Leishmania donovani |
| 7.4.2.1 | AAP7 | - |
Trypanosoma cruzi |
| 7.4.2.1 | amino acid permease 7 | - |
Leishmania donovani |
| 7.4.2.1 | lysine permease | - |
Leishmania donovani |
| 7.4.2.1 | lysine permease | - |
Trypanosoma cruzi |
| 7.4.2.1 | lysine-specific permease | - |
Leishmania donovani |
| 7.4.2.1 | lysine-specific permease | - |
Trypanosoma cruzi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.4.2.1 | 28 | - |
assay at | Trypanosoma cruzi |
| 7.4.2.1 | 30 | - |
assay at | Leishmania donovani |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.4.2.1 | 4.5 | - |
recombinant enzyme | Leishmania donovani |
| 7.4.2.1 | 7 | - |
assay at | Trypanosoma cruzi |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.4.2.1 | evolution | the enzyme belongs to the large amino acid/auxin permease family | Leishmania donovani |
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