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Literature summary extracted from

  • Okazaki, N.; Adachi, M.; Tamada, T.; Kurihara, K.; Ooga, T.; Kamiya, N.; Kuramitsu, S.; Kuroki, R.
    Crystallization and preliminary neutron diffraction studies of ADP-ribose pyrophosphatase-I from Thermus thermophilus HB8 (2012), Acta Crystallogr. Sect. F, 68, 49-52.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.13 expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.13 in complex with alpha,beta-methyleneadenosine diphosphoribose, sitting drop vapor diffusion method, using 18% (w/v) PEG 4000, 0.1 M sodium acetate buffer pH 5.3, 20% (w/v) glycerol, 0.2 M ammonium sulfate, at 20°C Thermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.13 Mg2+ required Thermus thermophilus
3.6.1.13 Zn2+ required Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.13 Thermus thermophilus Q84CU3
-
-
3.6.1.13 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q84CU3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.13 ammonium sulfate precipitation, Toyopearl Phenyl-650M column chromatography, and Q Sepharose column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.13 ADP-ribose + H2O highly specific substrate Thermus thermophilus AMP + D-ribose 5-phosphate
-
 ?
3.6.1.13 ADP-ribose + H2O highly specific substrate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + D-ribose 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.13 ADP-ribose pyrophosphatase-I
-
 Thermus thermophilus
3.6.1.13 ADPRase-I
-
 Thermus thermophilus
3.6.1.13 Ndx4
-
 Thermus thermophilus