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Literature summary extracted from
- Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus (2011), Acta Crystallogr. Sect. F, 67, 51-53.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.99.2 | expression of C-terminally Strep-tagged TenA in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.5.99.2 | purified recombinant C-terminally Strep-tagged TenA, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein in 100 mM Tris-HCl buffer pH 8.0, is mixed with 0.002 l of reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris-HCl pH 8.5 and 28% w/v PEG 3350, 20°C, 7 days, X-ray diffraction structure determination and analysis at 2.60 A resolution, molecular replacement, modelling | Staphylococcus aureus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.2 | 26840 | - |
x * 26840, sequence calculation | Staphylococcus aureus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.99.2 | Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.99.2 | recombinant C-terminally Strep-tagged TenA from Escherichia coli strain BL21(DE3) by affinity chromatography | Staphylococcus aureus |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.99.2 | ? | x * 26840, sequence calculation | Staphylococcus aureus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.99.2 | TenA | - |
Staphylococcus aureus |
| 3.5.99.2 | thiaminase type II | - |
Staphylococcus aureus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.5.99.2 | metabolism | thiaminase type II, TenA, catalyzes the deamination of aminopyrimidines, including the cleavage of thiamine to 4-amino-5-hydroxymethyl-2-methylpyrimidine and 5-(2-hydroxyethyl)-4-methylthiazole in the metabolism of thiamine (vitamin B1), in Staphylococcus aureus | Staphylococcus aureus |
| 3.5.99.2 | additional information | putative active-site residues are Asp44, Cys137, Tyr167 and Glu208 | Staphylococcus aureus |
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Release 2023.1 (January 2023)
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