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Literature summary extracted from

  • Chan, S.; Giuroiu, I.; Chernishof, I.; Sawaya, M.R.; Chiang, J.; Gunsalus, R.P.; Arbing, M.A.; Perry, L.J.
    Apo and ligand-bound structures of ModA from the archaeon Methanosarcina acetivorans (2010), Acta Crystallogr. Sect. F, 66, 242-250.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.5 expression of His-tagged ModA in Escherichia coli strain BL21-Gold (DE3) Methanosarcina acetivorans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.3.2.5 ModA in the apoprotein conformation and in the molybdate-bound conformation, by sitting-drop and hanging-drop vapor-diffusion methods, mixing of 0.001 ml of protein solution containing 16-23 mg/ml protein with 0.001 ml reservoir solution, containing 1.4 M ammonium citrate pH 6.4 or 1.4 M ammonium citrate pH 6.0, 4 mM sodium sulfate for the apo-protein and 2.1 M ammonium sulfate and either 1% v/v or 3% v/v 2-propanol, and 4% v/v 2.4 M ammonium sulfate for the molybdate-bound protein, equilibration against 100 ml reservoir solution at room temperature for apo-odA and at 293 K for molybdate-bound ModA, 1 week, method optimization, X-ray diffraction structure determination and analysis of apo- and molybdate-bound-ModA at 1.95 A and 1.69 A resolution and at 2.25 A and 2.45 A resolutionm, respectively, molecular replacemen fails, usage of heavy metal labeling instead Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.5 ATP + H2O + molybdate/out Methanosarcina acetivorans
-
 ADP + phosphate + molybdate/in
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.5 Methanosarcina acetivorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.3.2.5 recombinant His-tagged ModA from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography and gel filtration Methanosarcina acetivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.5 ATP + H2O + molybdate/out
-
 Methanosarcina acetivorans ADP + phosphate + molybdate/in
-
 ?
7.3.2.5 ATP + H2O + molybdate/out molybdate-bound MaModA employs octahedral coordination of its substrate Methanosarcina acetivorans ADP + phosphate + molybdate/in
-
 ?

Subunits

EC Number Subunits Comment Organism
7.3.2.5 More ModABC consists of the ModA periplasmic solute-binding protein, the integral membrane-transport protein ModB and the ATP-binding and hydrolysis cassette protein ModC. Bilobal domain structure of ModA with two mixed alpha/beta domains linked by a hinge region and with a deep cleft between the two domains. Upon binding ligand one domain is rotated towards the other by a hinge-bending motion analogously to the Venus flytrap model of bacterial-type periplasmic binding proteins Methanosarcina acetivorans

Synonyms

EC Number Synonyms Comment Organism
7.3.2.5 ModABC
-
 Methanosarcina acetivorans

General Information

EC Number General Information Comment Organism
7.3.2.5 physiological function ModABC consists of the ModA periplasmic solute-binding protein, the integral membrane-transport protein ModB and the ATP-binding and hydrolysis cassette protein ModC Methanosarcina acetivorans