BRENDA - Enzyme Database

New family of biuret hydrolases involved in s-triazine ring metabolism

Cameron, S.; Durchschein, K.; Richman, J.; Sadowsky, M.; Wackett, L.; ACS Catal. 1, 1075-1082 (2011)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.84
expressed in Escherichia coli DH5alpha cells
Rhizobium leguminosarum bv. viciae
General Stability
EC Number
General Stability
Organism
3.5.1.84
potassium phosphate buffer yields optimal catalytic rates
Rhizobium leguminosarum bv. viciae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.84
N-ethylmaleimide
more than 90% loss of activity at 0.05 mM; more than 90% loss of activity at 0.5 mM
Rhizobium leguminosarum bv. viciae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.84
0.023
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.84
additional information
the addition of ZnSO4 or CoSO4 (0.05 mM and 1 mM) to enzyme assays gives only nominal increases in activity. No metal cofactors are detected
Rhizobium leguminosarum bv. viciae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.84
27121
-
x * 27121, calculated from amino acid sequence
Rhizobium leguminosarum bv. viciae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.84
biuret + H2O
Rhizobium leguminosarum bv. viciae
the enzyme acts uniquely on biuret physiologically
allophanic acid + NH3
-
-
ir
3.5.1.84
biuret + H2O
Rhizobium leguminosarum bv. viciae 3841
the enzyme acts uniquely on biuret physiologically
allophanic acid + NH3
-
-
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.84
Rhizobium leguminosarum bv. viciae
-
-
-
3.5.1.84
Rhizobium leguminosarum bv. viciae 3841
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.84
nickel affinity column chromatography
Rhizobium leguminosarum bv. viciae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.84
0.02
-
below 0.02 micromol/min/mg, using 1,1-dimethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-carbamimidoylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-ethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-hydroxyurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-methylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-methylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-prop-2-en-1-ylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 2-cyanoacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 2-methylpropanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 3-oxobutanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using acetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using butanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using dicarbonodithioimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using (E)-diazene-1,2-dicarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using ethanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using ethanethioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using ethyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using ethyl carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using formamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using hexanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using hydrazinecarbothioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using hydrazinecarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using N-carbamothioylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using N-hydroxydicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using N-nitrodicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using prop-2-enamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using propanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using sulfuric diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using thiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using urea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
3.5.1.84
0.04
-
using N-carbamoylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
3.5.1.84
0.17
-
using 1-formylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
3.5.1.84
6.08
-
using dicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
3.5.1.84
8.9
-
using biuret as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.84
(E)-diazene-1,2-dicarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
(E)-diazene-1,2-dicarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
3.5.1.84
1,1-dimethylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-carbamimidoylthiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-ethylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-formylurea + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-formylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-hydroxyurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-methylthiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-methylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-prop-2-en-1-ylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
2-cyanoacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
2-methylpropanamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
3-oxobutanamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
acetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
acetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
3.5.1.84
biuret + H2O
the enzyme acts uniquely on biuret physiologically
718459
Rhizobium leguminosarum bv. viciae
allophanic acid + NH3
-
-
-
ir
3.5.1.84
biuret + H2O
the enzyme acts uniquely on biuret physiologically
718459
Rhizobium leguminosarum bv. viciae 3841
allophanic acid + NH3
-
-
-
ir
3.5.1.84
butanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
carbamoylcarbamate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
dicarbonimidic diamide + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
dicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
dicarbonodithioimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
ethanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
ethanethioamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
ethyl acetate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
ethyl acetate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
3.5.1.84
ethyl carbamoylcarbamate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
formamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
hexanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
hydrazinecarbothioamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
hydrazinecarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-carbamothioylacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-carbamoylacetamide + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-carbamoylacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-hydroxydicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-nitrodicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
prop-2-enamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
propanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
sulfuric diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
thiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
urea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
urea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.84
?
x * 27121, calculated from amino acid sequence
Rhizobium leguminosarum bv. viciae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.84
4
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.84
8.5
-
-
Rhizobium leguminosarum bv. viciae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.84
4.5
11
about 50% activity at pH 4.5 and pH 11.0
Rhizobium leguminosarum bv. viciae
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.1.84
Rhizobium leguminosarum bv. viciae
calculated from amino acid sequence
-
5.7
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.84
expressed in Escherichia coli DH5alpha cells
Rhizobium leguminosarum bv. viciae
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.1.84
potassium phosphate buffer yields optimal catalytic rates
Rhizobium leguminosarum bv. viciae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.84
N-ethylmaleimide
more than 90% loss of activity at 0.05 mM; more than 90% loss of activity at 0.5 mM
Rhizobium leguminosarum bv. viciae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.84
0.023
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.84
additional information
the addition of ZnSO4 or CoSO4 (0.05 mM and 1 mM) to enzyme assays gives only nominal increases in activity. No metal cofactors are detected
Rhizobium leguminosarum bv. viciae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.84
27121
-
x * 27121, calculated from amino acid sequence
Rhizobium leguminosarum bv. viciae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.84
biuret + H2O
Rhizobium leguminosarum bv. viciae
the enzyme acts uniquely on biuret physiologically
allophanic acid + NH3
-
-
ir
3.5.1.84
biuret + H2O
Rhizobium leguminosarum bv. viciae 3841
the enzyme acts uniquely on biuret physiologically
allophanic acid + NH3
-
-
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.84
nickel affinity column chromatography
Rhizobium leguminosarum bv. viciae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.84
0.02
-
below 0.02 micromol/min/mg, using 1,1-dimethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-carbamimidoylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-ethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-hydroxyurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-methylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-methylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 1-prop-2-en-1-ylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 2-cyanoacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 2-methylpropanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using 3-oxobutanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using acetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using butanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using dicarbonodithioimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using (E)-diazene-1,2-dicarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using ethanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using ethanethioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using ethyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using ethyl carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using formamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using hexanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using hydrazinecarbothioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using hydrazinecarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using N-carbamothioylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using N-hydroxydicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using N-nitrodicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using prop-2-enamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using propanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using sulfuric diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using thiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C; below 0.02 micromol/min/mg, using urea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
3.5.1.84
0.04
-
using N-carbamoylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
3.5.1.84
0.17
-
using 1-formylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
3.5.1.84
6.08
-
using dicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
3.5.1.84
8.9
-
using biuret as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.84
(E)-diazene-1,2-dicarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
(E)-diazene-1,2-dicarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
3.5.1.84
1,1-dimethylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-carbamimidoylthiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-ethylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-formylurea + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-formylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-hydroxyurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-methylthiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-methylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
1-prop-2-en-1-ylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
2-cyanoacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
2-methylpropanamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
3-oxobutanamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
acetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
acetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
3.5.1.84
biuret + H2O
the enzyme acts uniquely on biuret physiologically
718459
Rhizobium leguminosarum bv. viciae
allophanic acid + NH3
-
-
-
ir
3.5.1.84
biuret + H2O
the enzyme acts uniquely on biuret physiologically
718459
Rhizobium leguminosarum bv. viciae 3841
allophanic acid + NH3
-
-
-
ir
3.5.1.84
butanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
carbamoylcarbamate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
dicarbonimidic diamide + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
dicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
dicarbonodithioimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
ethanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
ethanethioamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
ethyl acetate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
ethyl acetate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
3.5.1.84
ethyl carbamoylcarbamate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
formamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
hexanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
hydrazinecarbothioamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
hydrazinecarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-carbamothioylacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-carbamoylacetamide + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-carbamoylacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-hydroxydicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
N-nitrodicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
prop-2-enamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
propanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
sulfuric diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
thiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
urea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3.5.1.84
urea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.84
?
x * 27121, calculated from amino acid sequence
Rhizobium leguminosarum bv. viciae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.84
4
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.84
8.5
-
-
Rhizobium leguminosarum bv. viciae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.84
4.5
11
about 50% activity at pH 4.5 and pH 11.0
Rhizobium leguminosarum bv. viciae
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.1.84
Rhizobium leguminosarum bv. viciae
calculated from amino acid sequence
-
5.7
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.84
170
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.84
170
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C
Rhizobium leguminosarum bv. viciae