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Literature summary extracted from

  • Cameron, S.; Durchschein, K.; Richman, J.; Sadowsky, M.; Wackett, L.
    New family of biuret hydrolases involved in s-triazine ring metabolism (2011), ACS Catal., 1, 1075-1082.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.84 expressed in Escherichia coli DH5alpha cells Rhizobium leguminosarum bv. viciae

General Stability

EC Number General Stability Organism
3.5.1.84 potassium phosphate buffer yields optimal catalytic rates Rhizobium leguminosarum bv. viciae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.84 N-ethylmaleimide more than 90% loss of activity at 0.05 mM; more than 90% loss of activity at 0.5 mM Rhizobium leguminosarum bv. viciae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.84 0.023
-
 Biuret in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.84 additional information the addition of ZnSO4 or CoSO4 (0.05 mM and 1 mM) to enzyme assays gives only nominal increases in activity. No metal cofactors are detected Rhizobium leguminosarum bv. viciae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.84 27121
-
 x * 27121, calculated from amino acid sequence Rhizobium leguminosarum bv. viciae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.84 biuret + H2O Rhizobium leguminosarum bv. viciae the enzyme acts uniquely on biuret physiologically allophanic acid + NH3
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.84 Rhizobium leguminosarum bv. viciae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.84 nickel affinity column chromatography Rhizobium leguminosarum bv. viciae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using (E)-diazene-1,2-dicarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 1,1-dimethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 1-carbamimidoylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 1-ethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 1-hydroxyurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 1-methylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 1-methylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 1-prop-2-en-1-ylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 2-cyanoacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 2-methylpropanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using 3-oxobutanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using acetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using butanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using dicarbonodithioimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using ethanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using ethanethioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using ethyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using ethyl carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using formamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using hexanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using hydrazinecarbothioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using hydrazinecarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using N-carbamothioylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using N-hydroxydicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using N-nitrodicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using prop-2-enamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using propanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using sulfuric diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using thiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.02
-
 below 0.02 micromol/min/mg, using urea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.04
-
 using N-carbamoylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 0.17
-
 using 1-formylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 6.08
-
 using dicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae
3.5.1.84 8.9
-
 using biuret as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.84 (E)-diazene-1,2-dicarboxamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 1,1-dimethylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 1-carbamimidoylthiourea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 1-ethylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 1-formylurea + H2O
-
 Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 1-formylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 1-hydroxyurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 1-methylthiourea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 1-methylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 1-prop-2-en-1-ylurea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 2-cyanoacetamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 2-methylpropanamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 3-oxobutanamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 acetamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 biuret + H2O the enzyme acts uniquely on biuret physiologically Rhizobium leguminosarum bv. viciae allophanic acid + NH3
-
 ir
3.5.1.84 butanediamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 carbamoylcarbamate + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 dicarbonimidic diamide + H2O
-
 Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 dicarbonimidic diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 dicarbonodithioimidic diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 ethanediamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 ethanethioamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 ethyl acetate + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 ethyl carbamoylcarbamate + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 formamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 hexanediamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 hydrazinecarbothioamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 hydrazinecarboxamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 N-carbamothioylacetamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 N-carbamoylacetamide + H2O
-
 Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 N-carbamoylacetamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 N-hydroxydicarbonimidic diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 N-nitrodicarbonimidic diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 prop-2-enamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 propanediamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 sulfuric diamide + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 thiourea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?
3.5.1.84 urea + H2O low specific activity Rhizobium leguminosarum bv. viciae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.84 ? x * 27121, calculated from amino acid sequence Rhizobium leguminosarum bv. viciae

Synonyms

EC Number Synonyms Comment Organism
3.5.1.84 biuret hydrolase
-
 Rhizobium leguminosarum bv. viciae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.84 4
-
 Biuret in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.84 8.5
-
-
 Rhizobium leguminosarum bv. viciae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.84 4.5 11 about 50% activity at pH 4.5 and pH 11.0 Rhizobium leguminosarum bv. viciae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.84 Rhizobium leguminosarum bv. viciae calculated from amino acid sequence
-
 5.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.84 170
-
 Biuret in 0.1 M potassium phosphate buffer, pH 8.5, at 22°C Rhizobium leguminosarum bv. viciae