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Literature summary extracted from
- Gene sequence, bioinformatics and enzymatic characterization of alpha-amylase from Saccharomycopsis fibuligera KZ (2010), Protein J., 29, 355-364.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in Saccharomyces cerevisiae strain AH22, subcloning in Escherichia coli strain TG1 | Saccharomycopsis fibuligera |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.1 | extracellular | - |
Saccharomycopsis fibuligera | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 53000 | - |
x * 53000, recombinant enzyme, SDS-PAGE | Saccharomycopsis fibuligera |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Saccharomycopsis fibuligera | D4P4Y7 | - |
- |
| 3.2.1.1 | Saccharomycopsis fibuligera KZ | D4P4Y7 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | glycoprotein | the recombinant enzyme contains about 2.5% of carbohydrate | Saccharomycopsis fibuligera |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | recombinant enzyme from Saccharomyces cerevisiae to homogeneity | Saccharomycopsis fibuligera |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | amylopectin + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? |  |
| 3.2.1.1 | amylose + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | amylose + H2O | - |
Saccharomycopsis fibuligera KZ | ? | - |
? | |
| 3.2.1.1 | beta-cyclodextrin + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | beta-cyclodextrin + H2O | - |
Saccharomycopsis fibuligera KZ | ? | - |
? | |
| 3.2.1.1 | beta-limit dextrin + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | corn starch + H2O | boiled starch | Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | gamma-cyclodextrin + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | maltoheptaose + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | maltoheptaose + H2O | - |
Saccharomycopsis fibuligera KZ | ? | - |
? | |
| 3.2.1.1 | additional information | substrate specificity, overview | Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | additional information | substrate specificity, overview | Saccharomycopsis fibuligera KZ | ? | - |
? | |
| 3.2.1.1 | pullulan + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | pullulan + H2O | - |
Saccharomycopsis fibuligera KZ | ? | - |
? | |
| 3.2.1.1 | raw starch + H2O | the recombinant enzyme shows low degradation of raw starch in a concentration-dependent manner | Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | rice starch + H2O | boiled starch | Saccharomycopsis fibuligera | ? | - |
? | |
| 3.2.1.1 | soluble starch + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | ? | x * 53000, recombinant enzyme, SDS-PAGE | Saccharomycopsis fibuligera |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | More | the enzyme belongs to the glycoside hydrolase family 13, subfamily 1 | Saccharomycopsis fibuligera |
| 3.2.1.1 | Sfamy | - |
Saccharomycopsis fibuligera |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 40 | 50 | - |
Saccharomycopsis fibuligera |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 30 | 60 | activity range | Saccharomycopsis fibuligera |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 5 | 6 | - |
Saccharomycopsis fibuligera |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 3.9 | 7 | activity range | Saccharomycopsis fibuligera |
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