EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.8 | expressed in Clostridium perfringens strains 13 and 13DELTA6 | Hathewaya histolytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.8 | 0.3 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | purified recombinant enzyme, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica | |
3.4.22.8 | 0.34 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | commercial enzyme preparation, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.8 | 14929 | - |
1 * 38061 + 1 * 14929, purified recombinant enzyme, MALDI-TOF mass spectrometry | Hathewaya histolytica |
3.4.22.8 | 14947 | - |
1 * 38067 + 1 * 14947, commercial enzyme preparation, MALDI-TOF mass spectrometry | Hathewaya histolytica |
3.4.22.8 | 14957 | - |
1 * 38091 + 1 * 14957, purified recombinant enzyme, calculated from amino acid sequence | Hathewaya histolytica |
3.4.22.8 | 15000 | - |
1 * 38000 + 1 * 15000, SDS-PAGE | Hathewaya histolytica |
3.4.22.8 | 38000 | - |
1 * 38000 + 1 * 15000, SDS-PAGE | Hathewaya histolytica |
3.4.22.8 | 38061 | - |
1 * 38061 + 1 * 14929, purified recombinant enzyme, MALDI-TOF mass spectrometry | Hathewaya histolytica |
3.4.22.8 | 38067 | - |
1 * 38067 + 1 * 14947, commercial enzyme preparation, MALDI-TOF mass spectrometry | Hathewaya histolytica |
3.4.22.8 | 38091 | - |
1 * 38091 + 1 * 14957, purified recombinant enzyme, calculated from amino acid sequence | Hathewaya histolytica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.8 | Hathewaya histolytica | P09870 | - |
- |
3.4.22.8 | Hathewaya histolytica ATCC 19401 | P09870 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.8 | ammonium sulfate precipitation, hydroxyapatite column chromatography, and benzamidine-Sepharose column chromatography | Hathewaya histolytica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.22.8 | 0.022 | - |
commercial enzyme preparation, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
3.4.22.8 | 0.039 | - |
purified recombinant enzyme, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
3.4.22.8 | 0.119 | - |
culture supernatant, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
3.4.22.8 | 61.2 | - |
recombinant enzyme after 515fold purification, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.8 | acyl-L-lysine-4-nitroanilide + H2O | - |
Hathewaya histolytica | acyl-L-lysine + 4-nitroaniline | - |
? | |
3.4.22.8 | acyl-L-lysine-4-nitroanilide + H2O | - |
Hathewaya histolytica ATCC 19401 | acyl-L-lysine + 4-nitroaniline | - |
? | |
3.4.22.8 | Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O | the enzyme exhibits strict specificity for arginine at the P1 position, the enzyme displays 2fold higher specific activity with N-benzoyl-L-arginine-4-nitroanilide compared to acyl-L-lysine-4-nitroanilide | Hathewaya histolytica | Nalpha-benzoyl-L-arginine + 4-nitroaniline | - |
? | |
3.4.22.8 | Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O | the enzyme exhibits strict specificity for arginine at the P1 position, the enzyme displays 2fold higher specific activity with N-benzoyl-L-arginine-4-nitroanilide compared to acyl-L-lysine-4-nitroanilide | Hathewaya histolytica ATCC 19401 | Nalpha-benzoyl-L-arginine + 4-nitroaniline | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.8 | heterodimer | 1 * 38000 + 1 * 15000, SDS-PAGE | Hathewaya histolytica |
3.4.22.8 | heterodimer | 1 * 38061 + 1 * 14929, purified recombinant enzyme, MALDI-TOF mass spectrometry | Hathewaya histolytica |
3.4.22.8 | heterodimer | 1 * 38067 + 1 * 14947, commercial enzyme preparation, MALDI-TOF mass spectrometry | Hathewaya histolytica |
3.4.22.8 | heterodimer | 1 * 38091 + 1 * 14957, purified recombinant enzyme, calculated from amino acid sequence | Hathewaya histolytica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.8 | CLO | - |
Hathewaya histolytica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.8 | 38.8 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | commercial enzyme preparation, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica | |
3.4.22.8 | 69.5 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | purified recombinant enzyme, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.8 | 116 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | commercial enzyme preparation, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica | |
3.4.22.8 | 234 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | purified recombinant enzyme, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |