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Literature summary extracted from
- Purification and characterization of a novel leucine aminopeptidase from the earthworm Eisenia foetida (2011), Process Biochem., 46, 1641-1648.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.1 | 1,10-phenanthroline | 3.3% activity at 0.2 mM | Eisenia fetida |  |
| 3.4.11.1 | bestatin | 16.4% activity at 0.05 mM | Eisenia fetida | |
| 3.4.11.1 | Ca2+ | partially inactivated at 1 mM | Eisenia fetida | |
| 3.4.11.1 | Cd2+ | strongly inactivated at 1 mM | Eisenia fetida | |
| 3.4.11.1 | Cu2+ | strongly inactivated at 1 mM | Eisenia fetida | |
| 3.4.11.1 | EDTA | 40.7% activity at 5 mM, 6.7% activity after extensive dialysis against 5 mM, restored to 60.1% with 0.05 mM Zn2+ | Eisenia fetida | |
| 3.4.11.1 | EGTA | 43.7% activity at 5 mM | Eisenia fetida | |
| 3.4.11.1 | Fe2+ | strongly inactivated at 1 mM | Eisenia fetida | |
| 3.4.11.1 | Leu | 41.5% activity at 1 mM | Eisenia fetida | |
| 3.4.11.1 | Mn2+ | partially inactivated at 1 mM | Eisenia fetida | |
| 3.4.11.1 | Phe | 33.5% activity at 1 mM | Eisenia fetida | |
| 3.4.11.1 | Trp | 40.7% activity at 1 mM | Eisenia fetida | |
| 3.4.11.1 | Zn2+ | partially inactivated at 1 mM | Eisenia fetida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.1 | 0.0084 | - |
L-arginine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 0.015 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 0.019 | - |
L-valine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 0.025 | - |
L-proline-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 0.054 | - |
L-alanine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 0.055 | - |
L-tyrosine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 0.39 | - |
glycine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.1 | Ba2+ | slightly stimulated at 1 mM (111.6% activity) | Eisenia fetida | |
| 3.4.11.1 | Mg2+ | slightly stimulated at 1 mM (122.7% activity) | Eisenia fetida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 98000 | - |
1 * 98000, SDS-PAGE, gel filtration | Eisenia fetida |
| 3.4.11.1 | 100000 | - |
gel filtration | Eisenia fetida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.1 | Eisenia fetida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.1 | ammonium sulfate precipitation, hydrophobic interaction chromatography, gel filtration, ion exchange chromatography (DEAE), hydroxyapatite chromatography, filtration through Heparin Sepharose | Eisenia fetida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 0.958 | - |
substrate L-leucine-4-methylcoumaryl-7-amide, pH 7.0, 37°C | Eisenia fetida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.1 | glycine-4-methylcoumaryl-7-amide + H2O | 3.9% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | glycine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.1 | L-alanine-4-methylcoumaryl-7-amide + H2O | 32.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-alanine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.1 | L-arginine-4-methylcoumaryl-7-amide + H2O | 62.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.1 | L-leucine-4-methylcoumaryl-7-amide + H2O | - |
Eisenia fetida | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.1 | L-proline-4-methylcoumaryl-7-amide + H2O | 1.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-proline + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.1 | L-tyrosine-4-methylcoumaryl-7-amide + H2O | 8.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-tyrosine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.1 | L-valine-4-methylcoumaryl-7-amide + H2O | 2.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-valine + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.1 | monomer | 1 * 98000, SDS-PAGE, gel filtration | Eisenia fetida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.1 | leucine aminopeptidase | - |
Eisenia fetida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 45 | - |
- |
Eisenia fetida |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 4 | 35 | 98% activity after 1.5h, sharp decrease above 45°C, less than 4% activity after 1.5h at 50°C | Eisenia fetida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.1 | 1.3 | - |
L-proline-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 1.5 | - |
L-valine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 12.1 | - |
L-arginine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 13.8 | - |
L-tyrosine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 34.6 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 38.7 | - |
L-alanine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 45.6 | - |
glycine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 7 | - |
- |
Eisenia fetida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 5.5 | 9 | - |
Eisenia fetida |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 6.5 | 9 | lose activity rapidly below pH 6.0 or above pH 9.0 during incubation at 4°C for 24h | Eisenia fetida |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.1 | 50 | - |
L-proline-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 80 | - |
L-valine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 120 | - |
glycine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 250 | - |
L-tyrosine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 720 | - |
L-alanine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 1400 | - |
L-arginine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
| 3.4.11.1 | 2300 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
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