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Literature summary extracted from

  • Kopparapu, N.; Liu, Z.; Fei, F.; Yan, Q.; Jiang, Z.
    Purification and characterization of a chitinase (sAMC) with antifungal activity from seeds of Astragalus membranaceus (2011), Process Biochem., 46, 1370-1374.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 35500
-
 1 * 35500, SDS-PAGE Astragalus membranaceus
3.2.1.14 36900
-
 gel filtration Astragalus membranaceus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Astragalus membranaceus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.14 glycoprotein sAMC is a glycoprotein with 6.1% neutral sugars Astragalus membranaceus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme from seeds, by ammonium sulfate fractionation, chitin affinity chromatography, and gel filtration Astragalus membranaceus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 seed
-
 Astragalus membranaceus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 colloidal chitin + H2O
-
 Astragalus membranaceus N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetyl D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 35500, SDS-PAGE Astragalus membranaceus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 endochitinase
-
 Astragalus membranaceus
3.2.1.14 More sAMC belongs to glycosyl hydrolase family 19 chitinases which are mostly endochitinases Astragalus membranaceus
3.2.1.14 sAMC
-
 Astragalus membranaceus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 60
-
-
 Astragalus membranaceus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 40
-
 purified enzyme, half-life is 1190 min Astragalus membranaceus
3.2.1.14 50
-
 purified enzyme, half-life is 665.2 min Astragalus membranaceus
3.2.1.14 60
-
 purified enzyme, stable up to, half-life is 503 min Astragalus membranaceus
3.2.1.14 65
-
 purified enzyme, half-life is 150.4 min Astragalus membranaceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Astragalus membranaceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 8 activity range Astragalus membranaceus

General Information

EC Number General Information Comment Organism
3.2.1.14 physiological function sAMC has a role in conversion of chitin wastes into useful products. It also exhibits antifungal activity against Trichoderma viride, Botrytis cinerea, Fusarium oxysporum and Fusarium solani Astragalus membranaceus