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Literature summary extracted from

  • Harbut, M.B.; Velmourougane, G.; Dalal, S.; Reiss, G.; Whisstock, J.C.; Onder, O.; Brisson, D.; McGowan, S.; Klemba, M.; Greenbaum, D.C.
    Bestatin-based chemical biology strategy reveals distinct roles for malaria M1- and M17-family aminopeptidases (2011), Proc. Natl. Acad. Sci. USA, 108, E526-E534.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.20 amino acid residue 192-1085 expressed in Escherichia coli Plasmodium falciparum
3.4.11.20 His-tagged protein (amino acid residue 79-605) expressed in Escherichia coli Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.20
-
 Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.20 (2S,17S,20S,21R)-21-amino-5-(4-benzoylbenzyl)-2-[4-(hex-5-ynoylamino)butyl]-20-hydroxy-17-(naphthalen-2-ylmethyl)-4,7,16,19-tetraoxo-22-phenyl-9,12-dioxa-3,6,15,18-tetraazadocosan-1-amide
-
 Plasmodium falciparum
3.4.11.20 N-[(2-[2-[(N-[(2S,3R)-3-amino-4-[4-(benzyloxy)phenyl]-2-hydroxybutanoyl]-L-alanyl)amino]ethoxy]ethoxy)acetyl]-4-benzoylphenylalanyl-N6-hex-5-ynoyl-L-lysinamide
-
 Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.20 343000
-
 gel filtration Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.20 Plasmodium falciparum
-
-
-
3.4.11.20 Plasmodium falciparum Q8IL11
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.20
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.20 L-leucyl-7-amido-4-methylcoumarin + H2O
-
 Plasmodium falciparum L-leucine + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.20 homohexamer 343000, gel filtration (357000 calculated) Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
3.4.11.20 aminopeptidase N
-
 Plasmodium falciparum
3.4.11.20 Leucyl aminopeptidase
-
 Plasmodium falciparum
3.4.11.20 metallo-aminopeptidase M1
-
 Plasmodium falciparum
3.4.11.20 Pf-LAP
-
 Plasmodium falciparum
3.4.11.20 PfA-M1
-
 Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.20 0.000029
-
 (2S,17S,20S,21R)-21-amino-5-(4-benzoylbenzyl)-2-[4-(hex-5-ynoylamino)butyl]-20-hydroxy-17-(naphthalen-2-ylmethyl)-4,7,16,19-tetraoxo-22-phenyl-9,12-dioxa-3,6,15,18-tetraazadocosan-1-amide pH 7.5, temperature not specified in the publication Plasmodium falciparum
3.4.11.20 0.00026
-
 N-[(2-[2-[(N-[(2S,3R)-3-amino-4-[4-(benzyloxy)phenyl]-2-hydroxybutanoyl]-L-alanyl)amino]ethoxy]ethoxy)acetyl]-4-benzoylphenylalanyl-N6-hex-5-ynoyl-L-lysinamide pH 7.5, temperature not specified in the publication Plasmodium falciparum
3.4.11.20 0.00033
-
 (2S,17S,20S,21R)-21-amino-5-(4-benzoylbenzyl)-2-[4-(hex-5-ynoylamino)butyl]-20-hydroxy-17-(naphthalen-2-ylmethyl)-4,7,16,19-tetraoxo-22-phenyl-9,12-dioxa-3,6,15,18-tetraazadocosan-1-amide pH 7.5, temperature not specified in the publication Plasmodium falciparum
3.4.11.20 0.004
-
 N-[(2-[2-[(N-[(2S,3R)-3-amino-4-[4-(benzyloxy)phenyl]-2-hydroxybutanoyl]-L-alanyl)amino]ethoxy]ethoxy)acetyl]-4-benzoylphenylalanyl-N6-hex-5-ynoyl-L-lysinamide pH 7.5, temperature not specified in the publication Plasmodium falciparum

General Information

EC Number General Information Comment Organism
3.4.11.20 malfunction inhibition caused swelling of the parasite digestive vacuole and prevented proteolysis of hemoglobin derived oligopeptides, likely starving the parasite resulting in death Plasmodium falciparum
3.4.11.20 malfunction inhibition is lethal to parasites early in the life cycle, prior to the onset of hemoglobin degradation Plasmodium falciparum