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Literature summary extracted from

  • Chen, Y.; Patel, N.A.; Crombie, A.; Scrivens, J.H.; Murrell, J.C.
    Bacterial flavin-containing monooxygenase is trimethylamine monooxygenase (2011), Proc. Natl. Acad. Sci. USA, 108, 17791-17796.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.148 expressed in Escherichia coli BLR(DE3) pLysS cells Ruegeria pomeroyi
1.14.13.148 expressed in Escherichia coli BLR(DE3) pLysS cells Roseovarius sp.
1.14.13.148 expressed in Escherichia coli BLR(DE3) pLysS cells Candidatus Pelagibacter ubique
1.14.13.148 expressed in Escherichia coli BLR(DE3) pLysS cells Methylocella silvestris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.148 0.0094
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
1.14.13.148 0.0103
-
 Dimethylsulfide recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
1.14.13.148 0.0208
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Ruegeria pomeroyi
1.14.13.148 0.0216
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Roseovarius sp.
1.14.13.148 0.0275
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Candidatus Pelagibacter ubique
1.14.13.148 0.0282
-
 Methimazole recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
1.14.13.148 0.0285
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Candidatus Pelagibacter ubique
1.14.13.148 0.0357
-
 dimethylaniline recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
1.14.13.148 0.0897
-
 N,N-dimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
1.14.13.148 3.139
-
 cysteamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
1.14.13.148 3.575
-
 DMSO recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.148 Candidatus Pelagibacter ubique
-
-
-
1.14.13.148 Candidatus Pelagibacter ubique HTCC1002
-
-
-
1.14.13.148 Candidatus Pelagibacter ubique HTCC7211
-
-
-
1.14.13.148 Methylocella silvestris
-
-
-
1.14.13.148 no activity in Dinoroseobacter shibae
-
-
-
1.14.13.148 no activity in Oceanicola batsensis
-
-
-
1.14.13.148 no activity in Roseobacter sp.
-
-
-
1.14.13.148 no activity in Roseobacter sp. SK209-2-6
-
-
-
1.14.13.148 no activity in Sagittula stellata
-
-
-
1.14.13.148 Roseovarius sp.
-
-
-
1.14.13.148 Roseovarius sp. 217
-
-
-
1.14.13.148 Ruegeria pomeroyi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.148 cysteamine + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
1.14.13.148 dimethylaniline + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
1.14.13.148 dimethylsulfide + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
1.14.13.148 DMSO + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
1.14.13.148 methimazole + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
1.14.13.148 additional information no activity with N-monomethylamine, glutathione, and cysteine Methylocella silvestris ?
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2
-
 Ruegeria pomeroyi N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2
-
 Roseovarius sp. N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2
-
 Candidatus Pelagibacter ubique N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2
-
 Methylocella silvestris N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2
-
 Candidatus Pelagibacter ubique HTCC7211 N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2
-
 Candidatus Pelagibacter ubique HTCC1002 N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2
-
 Roseovarius sp. 217 N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 N,N-dimethylamine + NADPH + H+ + O2
-
 Methylocella silvestris N,N-dimethylamine N-oxide + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.148 flavin-containing monooxygenase
-
 Ruegeria pomeroyi
1.14.13.148 flavin-containing monooxygenase
-
 Roseovarius sp.
1.14.13.148 flavin-containing monooxygenase
-
 Candidatus Pelagibacter ubique
1.14.13.148 flavin-containing monooxygenase
-
 Methylocella silvestris
1.14.13.148 FMO
-
 Ruegeria pomeroyi
1.14.13.148 FMO
-
 Roseovarius sp.
1.14.13.148 FMO
-
 Candidatus Pelagibacter ubique
1.14.13.148 FMO
-
 Methylocella silvestris
1.14.13.148 TMA monooxygenase
-
 Ruegeria pomeroyi
1.14.13.148 TMA monooxygenase
-
 Roseovarius sp.
1.14.13.148 TMA monooxygenase
-
 Candidatus Pelagibacter ubique
1.14.13.148 TMA monooxygenase
-
 Methylocella silvestris
1.14.13.148 TMM
-
 Ruegeria pomeroyi
1.14.13.148 TMM
-
 Roseovarius sp.
1.14.13.148 TMM
-
 Candidatus Pelagibacter ubique
1.14.13.148 TMM
-
 Methylocella silvestris

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.148 FAD
-
 Ruegeria pomeroyi
1.14.13.148 FAD
-
 Roseovarius sp.
1.14.13.148 FAD
-
 Candidatus Pelagibacter ubique
1.14.13.148 FAD
-
 Methylocella silvestris
1.14.13.148 NADPH required for activity Ruegeria pomeroyi
1.14.13.148 NADPH required for activity Roseovarius sp.
1.14.13.148 NADPH required for activity Candidatus Pelagibacter ubique
1.14.13.148 NADPH required for activity Methylocella silvestris

Expression

EC Number Organism Comment Expression
1.14.13.148 Methylocella silvestris the enzyme is absent during growth on methanol additional information
1.14.13.148 Methylocella silvestris the enzyme is highly expressed in N,N,N-trimethylamine-grown Methylocella silvestris up