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Literature summary extracted from

  • Wrase, R.; Scott, H.; Hilgenfeld, R.; Hansen, G.
    The Legionella HtrA homologue DegQ is a self-compartmentizing protease that forms large 12-meric assemblies (2011), Proc. Natl. Acad. Sci. USA, 108, 10490-10495.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B50 expressed in Escherichia coli KU98 cells Legionella fallonii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.B50 sitting drop vapor diffusion method Legionella fallonii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.B50 periplasm
-
 Legionella fallonii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B50 320000 440000 gel filtration Legionella fallonii

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B50 Legionella fallonii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B50 nickel-affinity column chromatography, gel filtration Legionella fallonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B50 beta-casein + H2O
-
 Legionella fallonii ?
-
 ?
3.4.21.B50 additional information DegQ does not degrade native bovine serum albumin and reductively unfolded lysozyme Legionella fallonii ?
-
 ?
3.4.21.B50 unfolded bovine serum albumin + H2O
-
 Legionella fallonii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B50 dodecamer 12 * 48000-50000, dynamic light-scattering, DegQ assembles into large, cage-like dodecamers that form independently of unfolded substrate proteins. Dodecamer-formation is essential for the degradation of substrate proteins but not for the chaperone activity of DegQ Legionella fallonii

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B50 DegQ
-
 Legionella fallonii