EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.22.2 | tarocystatin-papain complex and C-terminal cystatin-like extension-papain complex, hanging drop vapor diffusion method, the tarocystatin-papain complex is crystallized from a drop containing 15% PEG monomethyl ether 2000, 0.05 M sodium acetate trihydrate, pH 4.6, 0.1 M ammonium sulfate against a reservoir of 30% PEG monomethyl ether 2000, 0.1 M sodium acetate trihydrate, pH 4.6, and 0.2 M ammonium sulfate. The complex of CtE-papain is crystallized from a drop containing 0.05 M HEPES, pH 7.5, and 35% (v/v) 2-methyl-2,4-pentanediol against a reservoir of 0.1 M HEPES, pH 7.5, and 70% (v/v) 2-methyl-2,4-pentanediol | Carica papaya |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.2 | tarocystatin | the N-terminal cystatin domain (residues 1-98) of tarocystatin has inhibitory ability against papain | Carica papaya |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.2 | tarocystatin + H2O | Carica papaya | the C-terminal cystatin-like extension of tarocystatin is easily digested by papain | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.2 | Carica papaya | P00784 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.2 | latex | - |
Carica papaya | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.2 | tarocystatin + H2O | the C-terminal cystatin-like extension of tarocystatin is easily digested by papain | Carica papaya | ? | - |
? |