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Literature summary extracted from

  • Ohnuma, T.; Numata, T.; Osawa, T.; Mizuhara, M.; Lampela, O.; Juffer, A.H.; Skriver, K.; Fukamizo, T.
    A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis (2011), Planta, 234, 123-137.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene chiC, quantitative real-time PCR expression analysis, recombinant expression in Escherichia coli Arabidopsis thaliana
3.2.1.200 expression in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.14 purified recombinant AtChiC, sitting drop vapor diffusion method, mixing of 0.001 ml of 5 mg/ml protein in water with 0.001 ml of reservoir solution containing 100 mM trisodium citrate, pH 5.6, and 20% PEG 10000, 20°C, 5 months, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement Arabidopsis thaliana
3.2.1.200 to 2.0 A resolution, and docking simulation with substrate N,N',N'',N''',N''''-pentaacetylchitopentaose Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.200 30000
-
-
 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Arabidopsis thaliana Q0WT03 gene chiC
-
3.2.1.200 Arabidopsis thaliana O81862
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.200 proteolytic modification protein contains a signal sequence consisting of 24 amino acid residues at the N-terminus Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant enzyme from Escherichia coli Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 additional information quantitative real-time PCR expression analysis of gene chiC Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 glycol chitin + H2O
-
 Arabidopsis thaliana ?
-
 ?
3.2.1.14 additional information recombinant AtChiC hydrolyzes N-acetylglucosamine oligomers producing dimers from the non-reducing end of the substrates, mode of action toward the oligosaccharide substrates, overview. The enzyme catalyzes transglycosylation reaction in a low efficiency Arabidopsis thaliana ?
-
 ?
3.2.1.14 N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O the enzyme hydrolyzes from the non-reducing end Arabidopsis thaliana N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose equal amounts of N,N'-diacetylchitobiose and N,N',N'',N'''-tetraacetylchitotetraose and a lesser amount of N,N',N''-triacetylchitotriose are produced in the early stage of the reaction ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O the enzyme hydrolyzed N,N',N'',N''',N''''-pentaacetylchitopentaose from the non-reducing end, docking simulation, detailed overview Arabidopsis thaliana N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.200 additional information the enzyme hydrolyzes N-acetylglucosamine oligomers producing dimers from the nonreducing end of the substrates. It shows predominantly exo-splitting mode, but contains a slight endo-splitting activity and catalyzes an anomeric retention reaction. The enzyme catalyzes transglycosylation reaction in a low efficiency Arabidopsis thaliana ?
-
 ?
3.2.1.200 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Arabidopsis thaliana N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
-
 ?
3.2.1.200 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Arabidopsis thaliana N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
 ?
3.2.1.200 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Arabidopsis thaliana 2 N,N'-diacetylchitobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 More AtChiC adopts an (beta/alpha)8 fold with a small insertion domain composed of an alpha-helix and a five-stranded beta-sheet, secondary structure comparison, overview Arabidopsis thaliana
3.2.1.200 ? x * 39031, calculated, x * 30000, SDS-PAGE, mature protein Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 AtChiC
-
 Arabidopsis thaliana
3.2.1.14 class V chitinase
-
 Arabidopsis thaliana
3.2.1.200 At4g19810
-
 Arabidopsis thaliana
3.2.1.200 ChiC
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 37 40 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
 assay at Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
3.2.1.14 Arabidopsis thaliana gene chiC is induced by the plant stress-related hormones abscisic acid and jasmonic acid and by the stress resulting from the elicitor flagellin, NaCl, and osmosis up
3.2.1.200 Arabidopsis thaliana expression is induced by both biotic and abiotic stress up