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Literature summary extracted from

  • Huang, F.; Sung, P.; Do, Y.; Huang, P.
    Differential expression and functional characterization of the NADPH cytochrome P450 reductase genes from Nothapodytes foetida (2012), Plant Sci., 190, 16-23.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.83 expression in Spodoptera frugiperda SF9 cells, co-expression with NADPH:cytochrome P450 reductase NfCPR2 from Nothapodytes foetida and CPR2 from Arabidopsis thaliana Catharanthus roseus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.83 additional information microsomes isolated from insect cells co-expressing Nothapodytes foetida NfCPR2 and Catharanthus roseus geraniol 10-hydroxylase show enhanced the production of eriodictyol from naringenin approximately 11fold relative to control G10H-only insect cells, indicating the supportive role of NfCPR2 for G10H activity in insect cells. Addition of FAD and FMN up to 0.01 mM does not increase the supportive function of the NfCPR2 for G10H activity Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
 (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.83 Catharanthus roseus Q8VWZ7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Catharanthus roseus (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.83 G10H
-
 Catharanthus roseus
1.14.14.83 geraniol 10-hydroxylase
-
 Catharanthus roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.83 30
-
 assay at Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.83 8
-
 assay at Catharanthus roseus

General Information

EC Number General Information Comment Organism
1.14.14.83 evolution the enzyme belongs to the cytochrome-P450 monooxygenase family Catharanthus roseus
1.14.14.83 metabolism the enzyme is involved in the biosynthesis of secologanin from geraniol, overview Catharanthus roseus