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Literature summary extracted from
- CYP76M7 is an ent-cassadiene C11 alpha-hydroxylase defining a second multifunctional diterpenoid biosynthetic gene cluster in rice (2009), Plant Cell, 21, 3315-3325.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.112 | expressed in Escherichia coli strain C41 and in Sf21 insect cells | Oryza sativa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.112 | 0.039 | - |
ent-cassa-12,15-diene | in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication | Oryza sativa |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.112 | Oryza sativa | Q6YTE2 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.112 | 0.13 | - |
using ent-cassa-12,15-diene as substrate in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication | Oryza sativa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.112 | ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2 | specific substrate, C11alpha hydroxylation | Oryza sativa | 11-(S)-hydroxy-entcassa-12,15-diene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.112 | additional information | only trace amounts of hydroxylation (less than 1% conversion) is observed for ent-pimaradiene, ent-sandaracopimaradiene, ent-kaurene, ent-isokaurene, synpimaradiene, syn-stemarene, or syn-stemodene | Oryza sativa | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.112 | CYP76M7 | - |
Oryza sativa |
| 1.14.14.112 | ent-cassadiene C11alpha-hydroxylase | - |
Oryza sativa |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.112 | physiological function | the enzyme acts in the production of antifungal phytocassanes | Oryza sativa |
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Release 2023.1 (January 2023)
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