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Literature summary extracted from

  • Koellner, T.; Schnee, C.; Gershenzon, J.; Degenhardt, J.
    The variability of sesquiterpenes emitted from two Zea mays cultivars is controlled by allelic variation of two terpene synthase genes encoding stereoselective multiple product enzymes (2004), Plant Cell, 16, 1115-1131.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.101 expressed in Escherichia coli BL21 (DE) cells Zea mays
4.2.3.102 expressed in Escherichia coli BL21(DE3) cells Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.101 E391Q the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity Zea mays
4.2.3.101 E455V the mutation results in an increase of catalytic activity Zea mays
4.2.3.101 I479T the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity Zea mays
4.2.3.101 K402Q the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity Zea mays
4.2.3.101 T383S the mutation is located in the vicinity of the catalytic center but does not influence enzymatic activity Zea mays
4.2.3.101 W163R the mutation restores much of the wild type activity (albeit maintaining the increased concentration of (E)-beta-farnesene relative to that of sesquithujene and (S)-beta-bisabolene) Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.101 0.0009
-
 geranyl diphosphate 20 mM MgCl2, 0.2 mM MnCl2, 0.2 mM NaWO4, and 0.1 mM NaF, pH not specified in the publication, at 30°C Zea mays
4.2.3.101 0.0011
-
 (2E,6E)-farnesyl diphosphate 20 mM MgCl2, 0.2 mM MnCl2, 0.2 mM NaWO4, and 0.1 mM NaF, pH not specified in the publication, at 30°C Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.101 Mg2+ either Mg2+ or Mn2+ is required for activity, the optimum is 5 mM Mg2+ Zea mays
4.2.3.101 Mn2+ either Mg2+ or Mn2+ is required for activity, the optimum is 0.25 mM Mg2+ Zea mays
4.2.3.101 additional information no enzyme activity in the presence of Ca2+, Cd2+, Co2+, Cu2+, Ni2+, and Zn2+ Zea mays
4.2.3.102 Mg2+ either Mg2+ or Mn2+ is required for activity, the optimum is 5 mM Mg2+ Zea mays
4.2.3.102 Mn2+ either Mg2+ or Mn2+ is required for activity, the optimum is 0.25 mM Mg2+ Zea mays
4.2.3.102 additional information no enzyme activity in the presence of Ca2+, Cd2+, Co2+, Cu2+, Ni2+, and Zn2+ Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.101 63960
-
 calculated from amino acid sequence Zea mays
4.2.3.101 66000
-
 gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.101 Zea mays
-
 inbred line B73
-
4.2.3.102 Zea mays
-
 variety Delprim
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.101 Econopac 10DG column chromatography and Strep-Tactine affinity column chromatography Zea mays
4.2.3.102 Econopac 10DG column chromatography and Strep-Tactine affinity column chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.101 husk highest expression Zea mays
-
4.2.3.101 leaf ear leaf Zea mays
-
4.2.3.102 husk highest expression Zea mays
-
4.2.3.102 leaf ear leaf Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.101 (2E,6E)-farnesyl diphosphate
-
 Zea mays 7-epi-sesquithujene + diphosphate 7-epi-sesquithujene is the main product, the enzyme also produces (S)-beta-bisabolene, (E)-beta-farnesene, and the minor products sesquithujene, (Z)-alpha-bergamotene, sesquisabinene B, (E)-alpha-bergamotene, and sesquisabinene A ?
4.2.3.101 geranyl diphosphate
-
 Zea mays (S)-(-)-limonene + diphosphate main product is (S)-(-)-limonene, minor products are alpha-thujene, beta-myrcene, (+)-sabinene, (-)-sabinene, alpha-terpinene, (E)-beta-ocimene, beta-phellandrene, alpha-terpinolene, gamma-terpinene, sabinene hydrate, (R)-(-)-linalool, and (S)-(1)-linalool ?
4.2.3.102 (2E,6E)-farnesyl diphosphate
-
 Zea mays sesquithujene + diphosphate sesquithujene is the major product, the enzyme also produces (S)-beta-bisabolene, and the minor products 7-epi-sesquithujene, (Z)-alpha-bergamotene, (E)-alpha-bergamotene, sesquisabinene B, sesquisabinene A, and (E)-beta-farnesene ?
4.2.3.102 geranyl diphosphate
-
 Zea mays (S)-(-)-limonene + diphosphate main product is (S)-(-)-limonene, minor products are alpha-thujene, beta-myrcene, (+)-sabinene, (-)-sabinene, alpha-terpinene, (E)-beta-ocimene, beta-phellandrene, alpha-terpinolene, gamma-terpinene, sabinene hydrate, (R)-(-)-linalool, and (S)-(1)-linalool ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.101 TPS4
-
 Zea mays
4.2.3.101 TPS4-B73
-
 Zea mays
4.2.3.102 TPS5
-
 Zea mays
4.2.3.102 TPS5-Del1
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.101 7
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.101 6.3 8.2
-
 Zea mays