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Literature summary extracted from

  • Kang, J.; Ju, H.; Sohn, W.; Na, B.
    Molecular cloning and characterization of a M17 leucine aminopeptidase of Cryptosporidium parvum (2011), Parasitology, 138, 682-690.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 His-tagged protein expressed in Escherichia coli M15 [pREP4] Cryptosporidium parvum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline 7.2% residual activity at 1 mM Cryptosporidium parvum
3.4.11.1 bestatin 0.4% residual activity at 0.001 mM Cryptosporidium parvum
3.4.11.1 EDTA 6.7% residual activity at 1 mM Cryptosporidium parvum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.102
-
L-leucine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.1092
-
L-methionine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.2396
-
L-alanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.245
-
L-phenylalanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.3641
-
L-lysine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Co2+ 47.8fold activity increase at 1 mM Cryptosporidium parvum
3.4.11.1 Mg2+ 10.9fold activity increase at 1 mM Cryptosporidium parvum
3.4.11.1 Mn2+ 64.3fold activity increase at 1 mM Cryptosporidium parvum
3.4.11.1 Zn2+ 12.1fold activity increase at 1 mM Cryptosporidium parvum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 58000
-
x * 58000, SDS-PAGE Cryptosporidium parvum

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Cryptosporidium parvum Q5CRI9
-
-
3.4.11.1 Cryptosporidium parvum Iowa II Q5CRI9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 immobilized metal ion affinity chromatography (Ni2+) Cryptosporidium parvum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-alanine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum L-alanine + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-alanine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum Iowa II L-alanine + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-leucine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum L-leucine + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-leucine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum Iowa II L-leucine + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-lysine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum L-lysine + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-lysine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum Iowa II L-lysine + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-methionine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum L-methionine + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-methionine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum Iowa II L-methionine + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-phenylalanine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum L-phenylalanine + 7-amino-4-methylcoumarin
-
?
3.4.11.1 L-phenylalanine-4-methylcoumaryl-7-amide + H2O
-
Cryptosporidium parvum Iowa II L-phenylalanine + 7-amino-4-methylcoumarin
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 58000, SDS-PAGE Cryptosporidium parvum

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 M17 leucine aminopeptidase
-
Cryptosporidium parvum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 0.002
-
L-lysine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.01
-
L-alanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.075
-
L-phenylalanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.085
-
L-methionine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.107
-
L-leucine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8.5
-
broad pH optimal range: pH 7.5-9.5 Cryptosporidium parvum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.1 additional information
-
highly unstable in acidic pHs, but relatively stable at neutral and alkaline conditions Cryptosporidium parvum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.1 0.0054
-
L-lysine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.0417
-
L-alanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.3061
-
L-phenylalanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 0.7784
-
L-methionine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
3.4.11.1 1.049
-
L-leucine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum