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Literature summary extracted from

  • Graham, J.E.; Clark, M.E.; Nadler, D.C.; Huffer, S.; Chokhawala, H.A.; Rowland, S.E.; Blanch, H.W.; Clark, D.S.; Robb, F.T.
    Identification and characterization of a multidomain hyperthermophilic cellulase from an archaeal enrichment (2011), Nat. Commun., 2, 375.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression in Escherichia coli BL21 (DE3) and Rosetta cells Desulfurococcaceae archaeon EBI-244

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 94000
-
 x * 94000, SDS-PAGE Desulfurococcaceae archaeon EBI-244

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.4 additional information Desulfurococcaceae archaeon EBI-244 EBI-244 is active on a range of high molecular weight carbohydrate substrates containing beta-1,4-linked glucose, including carboxymethyl cellulose, Avicel, and filter paper, it is active toward 4-nitrophenyl-cellobioside but inactive toward 4-nitrophenyl-glucoside ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Desulfurococcaceae archaeon EBI-244 F6M085 isolated from a sample of a circumneutral geothermal pool at 94 °C
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 4-nitrophenyl cellobioside + H2O
-
 Desulfurococcaceae archaeon EBI-244 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Desulfurococcaceae archaeon EBI-244 ?
-
 ?
3.2.1.4 additional information EBI-244 is active on a range of high molecular weight carbohydrate substrates containing beta-1,4-linked glucose, including carboxymethyl cellulose, Avicel, and filter paper, it is active toward 4-nitrophenyl-cellobioside but inactive toward 4-nitrophenyl-glucoside Desulfurococcaceae archaeon EBI-244 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 94000, SDS-PAGE Desulfurococcaceae archaeon EBI-244
3.2.1.4 More a multidomain cellulase Desulfurococcaceae archaeon EBI-244

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 More the enzyme is a a member of the TIM barrel glycosyl hydrolase superfamily (beta/alpha)8 Desulfurococcaceae archaeon EBI-244

General Information

EC Number General Information Comment Organism
3.2.1.4 additional information EBI-244 is a multidomain cellulase from a hyperthermophilic archaeon Desulfurococcaceae archaeon EBI-244