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Literature summary extracted from

  • Martin, N.; Christensen, Q.H.; Mansilla, M.C.; Cronan, J.E.; de Mendoza, D.
    A novel two-gene requirement for the octanoyltransfer reaction of Bacillus subtilis lipoic acid biosynthesis (2011), Mol. Microbiol., 80, 335-349.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.181 gene lipM, complements a lipB-defective Escherichia coli mutant strain Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.181 additional information construction of strain NM57 in which lipM is replaced with a kanamycin-resistance determinant. Gene lipM is placed under control of a xylose-inducible promoter PxylA is introduced into the DELTAlipM strain NM57 giving the lipM amyE::PxylA-lipM strain NM08. LipM can be functionally replaced by expression of Escherichia coli lipB, expression of Escherichia coli lipB allows growth of Bacillus subtilis DELTAlipL or DELATlipM strains in the absence of supplements. In contrast, growth of an Escherichia coli DELTAlipB strain can be complemented with lipM, but not lipL Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.204 31420
-
 calculated from amino acid sequence Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.181 an octanoyl-[acyl-carrier protein] + a protein Bacillus subtilis
-
 a protein N6-(octanoyl)lysine + an [acyl-carrier protein]
-
 ?
2.8.1.8 protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine Bacillus subtilis
-
 protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.181 Bacillus subtilis
-
 gene lipM
-
2.3.1.204 Bacillus subtilis P39648
-
-
2.8.1.8 Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.181 an octanoyl-[acyl-carrier protein] + a protein
-
 Bacillus subtilis a protein N6-(octanoyl)lysine + an [acyl-carrier protein]
-
 ?
2.3.1.181 an octanoyl-[acyl-carrier protein] + a protein acyl-enzyme intermediate mechanism Bacillus subtilis a protein N6-(octanoyl)lysine + an [acyl-carrier protein]
-
 ?
2.8.1.8 protein N6-(octanoyl)lysine + sulfur + S-adenosyl-L-methionine
-
 Bacillus subtilis protein N6-(lipoyl)lysine + L-methionine + 5'-deoxyadenosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.181 ACP:protein-N-octanoyltransferase
-
 Bacillus subtilis
2.3.1.181 LipM
-
 Bacillus subtilis
2.3.1.181 octanoyl-acyl carrier protein:protein-N-octanoyltransferase
-
 Bacillus subtilis
2.3.1.204 LIPL
-
 Bacillus subtilis
2.3.1.204 octanoyl-[GcvH]:E2 amidotransferase
-
 Bacillus subtilis
2.3.1.204 ywfL
-
 Bacillus subtilis
2.8.1.8 LipA
-
 Bacillus subtilis
2.8.1.8 lipoate synthase
-
 Bacillus subtilis

General Information

EC Number General Information Comment Organism
2.3.1.181 malfunction DELTAlipL strains are unable to synthesize lipoic acid despite the presence of LipM and the sulfur insertion enzyme, LipA, which should suffice for lipoic acid biosynthesis. Strain NM57 DELTAlipM is auxotrophic for lipoic acid when grown in minimal medium but grew as well as the wild type strain JH642 in the presence of lipoic acid Bacillus subtilis
2.3.1.181 physiological function LipM is required for modification of lipoyl domains by the biosynthetic pathway, it is responsible for octanoyl transfer in vivo requirement for LipL. But LipM is only required for the endogenous lipoylation pathway, whereas LipL also plays a role in lipoic acid scavenging Bacillus subtilis
2.8.1.8 metabolism the enzyme is involved in lipoic acid synthesis Bacillus subtilis