Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, Y.; Cao, H.; Yu, H.; Chen, Y.; Lau, K.; Qu, J.; Thon, V.; Sugiarto, G.; Chen, X.
    Identifying selective inhibitors against the human cytosolic sialidase NEU2 by substrate specificity studies (2011), Mol. Biosyst., 7, 1060-1072.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 isozyme NEU2, DNA and amino acid sequence determination and analysis, overexpression as soluble active enzyme in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.18 2,6-anhydro-3,5-dideoxy-5-glycolylamido-D-glycero-D-galactonon-2-enonic acid
-
 Homo sapiens
3.2.1.18 2,6-anhydro-3-deoxy-D-glycero-D-galacto-non-2-enonic acid
-
 Homo sapiens
3.2.1.18 2,6-anhydro-5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-D-galacto-non-2-enonic acid
-
 Homo sapiens
3.2.1.18 5-acetamido-2,6-anhydro-3,5-dideoxy-D-glycero-D-galactonon-2-enonic acid
-
 Homo sapiens
3.2.1.18 Ca2+
-
 Homo sapiens
3.2.1.18 Co2+
-
 Homo sapiens
3.2.1.18 Cu2+ 95% inhibition at 10 mM Homo sapiens
3.2.1.18 EDTA 20% inhibition at 1 mM, 54% at 10 mM Homo sapiens
3.2.1.18 Li+ 9% inhibition at 10 mM Homo sapiens
3.2.1.18 Mg2+
-
 Homo sapiens
3.2.1.18 Mn2+ 28% inhibition at 10 mM Homo sapiens
3.2.1.18 additional information design of a sialidase inhibitor selective against human NEU2, inhibitor docking using also the crystal structure of the enzyme, overview Homo sapiens
3.2.1.18 zanamivir
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.18 cytosol
-
 Homo sapiens 5829
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Homo sapiens
-
 isozyme NEU2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.18 glycoprotein one potential N-linked glycosylation site Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 (N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
-
 Homo sapiens N-glycolylneuraminic acid + 4-nitrophenol + ?
-
 ?
3.2.1.18 (N-glycolylneuraminic acid)(alpha2,6)Galbeta-p-nitrophenol + H2O
-
 Homo sapiens N-glycolylneuraminic acid + 4-nitrophenol + ?
-
 ?
3.2.1.18 2'-(4-methyl-umbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
-
 Homo sapiens 4-methyl-umbelliferol + alpha-D-N-acetylneuraminic acid
-
 ?
3.2.1.18 5-acetylneuraminic acid-(alpha2,3)Galbeta-4-nitrophenol + H2O
-
 Homo sapiens N-acetylneuraminic acid + 4-nitrophenol + ?
-
 ?
3.2.1.18 5-acetylneuraminic acid-(alpha2,6)Galbeta-4-nitrophenol + H2O
-
 Homo sapiens N-acetylneuraminic acid + 4-nitrophenol + ?
-
 ?
3.2.1.18 5-azidoacetylneuraminic acid-(alpha2,3)Galbeta-4-nitrophenol + H2O
-
 Homo sapiens N-azidoacetylneuraminic acid + 4-nitrophenol + ?
-
 ?
3.2.1.18 5-azidoacetylneuraminic acid-(alpha2,6)Galbeta-4-nitrophenol + H2O
-
 Homo sapiens N-azidoacetylneuraminic acid + 4-nitrophenol + ?
-
 ?
3.2.1.18 5-fluoroacetylneuraminic acid-(alpha2,3)Galbeta-4-nitrophenol + H2O
-
 Homo sapiens N-fluoroacetylneuraminic acid + 4-nitrophenol + ?
-
 ?
3.2.1.18 5-fluoroacetylneuraminic acid-(alpha2,6)Galbeta-4-nitrophenol + H2O
-
 Homo sapiens N-fluoroacetylneuraminic acid + 4-nitrophenol + ?
-
 ?
3.2.1.18 additional information both alpha2-3- and alpha2-6-linked sialosides are suitable substrates for NEU2 although it cleaves alpha2-3-linked sialosides more efficiently. Twenty 4-nitrophenyl-tagged alpha(2,3)- or alpha(2,6)-linked sialyl galactosides containing different terminal sialic acid forms including common N-acetylneuraminic acid, non-human N-glycolylneuraminic acid, 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid, or their C5-derivatives are used as substrates, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 NEU2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.18 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.18 5.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.18 4.5 6.5 activity range, almost inactive at pH 4.0 and pH 7.0 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.18 0.0053
-
 pH 5.5, 37°C Homo sapiens zanamivir
3.2.1.18 0.0083
-
 pH 5.5, 37°C Homo sapiens 2,6-anhydro-5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-D-galacto-non-2-enonic acid
3.2.1.18 0.018
-
 pH 5.5, 37°C Homo sapiens 5-acetamido-2,6-anhydro-3,5-dideoxy-D-glycero-D-galactonon-2-enonic acid
3.2.1.18 0.034
-
 pH 5.5, 37°C Homo sapiens 2,6-anhydro-3,5-dideoxy-5-glycolylamido-D-glycero-D-galactonon-2-enonic acid
3.2.1.18 2.1
-
 pH 5.5, 37°C Homo sapiens 2,6-anhydro-3-deoxy-D-glycero-D-galacto-non-2-enonic acid