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Literature summary extracted from
- Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor (2010), Microb. Cell Fact., 9, 37.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.31.1 | molecular biology | purified enzyme can be used as an exogenous reagent to clear cellular extracts and improve protein purification | Staphylococcus aureus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.31.1 | the enzyme is cloned into an expression-secretion vector lacking its own signal peptide but being fused to a lactococcal sequence encoding a signal peptide. It is then fused to a lactococcal sequence encoding a signal peptide. Functional recombinant expression of the enzyme under the control of a lactococcal promoter, that is inducible by zinc starvation, in Lactococcus lactis subsp. cremoris model strain MG1363, the enzyme is secreted to the GM17v culture medium, expression method optimization, overview | Staphylococcus aureus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.31.1 | extracellular | the enzyme is secreted | Staphylococcus aureus | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.31.1 | Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.31.1 | recombinant enzyme from Lactococcus lactis cell culture medium by cation exchange chromatography | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.31.1 | 2000 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Staphylococcus aureus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.31.1 | Nuc | - |
Staphylococcus aureus |
| 3.1.31.1 | SNase | - |
Staphylococcus aureus |
| 3.1.31.1 | staphylococcal nuclease | - |
Staphylococcus aureus |
| 3.1.31.1 | thermonuclease | - |
Staphylococcus aureus |
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Release 2023.1 (January 2023)
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