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Literature summary extracted from

  • Lafond, M.; Tauzin, A.; Desseaux, V.; Bonnin, E.; Ajandouz, E.; Giardina, T.
    GH10 xylanase D from Penicillium funiculosum: Biochemical studies and xylooligosaccharide production (2011), Microb. Cell Fact., 10, 20.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xynD, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain X33 Talaromyces funiculosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information kinetics, overview Talaromyces funiculosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular the enzyme is secreted Talaromyces funiculosus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 41540
-
 x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation Talaromyces funiculosus
3.2.1.8 46000
-
 x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation Talaromyces funiculosus
3.2.1.8 64000
-
 x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation Talaromyces funiculosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Talaromyces funiculosus Q5ZNB1 gene xynD
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein the recombinant protein is N- and O-glycosylated Talaromyces funiculosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant XynD 1.9fold from Pichia pastoris strain X33 by ultrafiltration and gel filtration Talaromyces funiculosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 additional information
-
 substrate specificity with different arabinoxylans, overview Talaromyces funiculosus
3.2.1.8 3 3.9 purified recombinant enzyme, pH 5.0, 70°C Talaromyces funiculosus
3.2.1.8 50.4
-
 purified recombinant enzyme, substrate birchwood xylan, pH 5.0, 70°C Talaromyces funiculosus
3.2.1.8 115.9
-
 purified recombinant enzyme, substrate high viscosity arabinoxylan, pH 5.0, 70°C Talaromyces funiculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 arabinoxylan + H2O low to high viscosity arabinoxylans, and water-extractable wheat arabinoxylans with different ratio arabinose:xylose, overview Talaromyces funiculosus ?
-
 ?
3.2.1.8 beech wood xylan + H2O
-
 Talaromyces funiculosus ?
-
 ?
3.2.1.8 birch wood xylan + H2O
-
 Talaromyces funiculosus ?
-
 ?
3.2.1.8 additional information the catalytic efficiency increases slightly with increasing their chain length with a small difference of the XynD catalytic efficiency against the different xylooligosaccharides Talaromyces funiculosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation Talaromyces funiculosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endo-1,4-beta-xylanase
-
 Talaromyces funiculosus
3.2.1.8 GH10 xylanase D
-
 Talaromyces funiculosus
3.2.1.8 More XynD belongs to the GH10 family Talaromyces funiculosus
3.2.1.8 XynD
-
 Talaromyces funiculosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 80
-
-
 Talaromyces funiculosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70
-
 purified recombinant enzyme, 180 min, no loss of activity Talaromyces funiculosus
3.2.1.8 80
-
 purified recombinant enzyme, 10 min, loss of 80% activity Talaromyces funiculosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
-
 Talaromyces funiculosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Talaromyces funiculosus isoelectric focusing
-
 5.9

General Information

EC Number General Information Comment Organism
3.2.1.8 additional information XynD possesses four catalytic subsites with a high energy of interaction with the substrate and a fifth subsite with a small energy of interaction Talaromyces funiculosus