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Literature summary extracted from

  • Hoffmann, T.; Kurtzer, R.; Skowranek, K.; Kiessling, P.; Fridman, E.; Pichersky, E.; Schwab, W.
    Metabolic engineering in strawberry fruit uncovers a dormant biosynthetic pathway (2011), Metab. Eng., 13, 527-531.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.318 EGS1 expression in Fragaria x ananassa cv. Elsanta via transfection with Agrobacterium tumefaciens strain AGL0, downregulation of chalcone synthase via antisense construct in parallel Ocimum basilicum
1.1.1.319 IGS1 expression in Fragaria x ananassa cv. Elsanta via transfection with Agrobacterium tumefaciens strain AGL0, downregulation of chalcone synthase via antisense construct in parallel Petunia x hybrida

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.318 additional information recombinant Ocimum basilicum EGS transient overexpression in agroinfiltrated fruits leads to a substantial increase in the accumulation of chavicol and eugenol the endogenous phenylpropene pathway and the EGS protein compete for common substrates, specificity of EGS for the production of allyl phenols Ocimum basilicum
1.1.1.319 additional information recombinant Petunia hybrida IGS expression alone does not increase the concentration of anol or isoeugenol in agroinfiltrated strawberry fruits, concurrent silencing of CHS substantially enhances the yield of the two metabolites Petunia x hybrida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.318 4-coumaryl acetate + NADPH + H+ Ocimum basilicum
-
 chavicol + acetate + NADP+
-
 ?
1.1.1.318 coniferyl acetate + NADPH + H+ Ocimum basilicum
-
 eugenol + acetate + NADP+
-
 ?
1.1.1.318 additional information Ocimum basilicum specificity of EGS for the production of allyl phenols in heterologically EGS expressing Fragaria x ananassa fruits ?
-
 ?
1.1.1.319 4-coumaryl acetate + NADPH + H+ Petunia x hybrida
-
 t-anol + acetate + NADP+
-
 ?
1.1.1.319 coniferyl acetate + NADPH + H+ Petunia x hybrida
-
 isoeugenol + acetate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.318 Ocimum basilicum Q15GI4
-
-
1.1.1.319 Petunia x hybrida Q15GI3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.318 4-coumaryl acetate + NADPH + H+
-
 Ocimum basilicum chavicol + acetate + NADP+
-
 ?
1.1.1.318 coniferyl acetate + NADPH + H+
-
 Ocimum basilicum eugenol + acetate + NADP+
-
 ?
1.1.1.318 additional information specificity of EGS for the production of allyl phenols in heterologically EGS expressing Fragaria x ananassa fruits Ocimum basilicum ?
-
 ?
1.1.1.319 4-coumaryl acetate + NADPH + H+
-
 Petunia x hybrida t-anol + acetate + NADP+
-
 ?
1.1.1.319 coniferyl acetate + NADPH + H+
-
 Petunia x hybrida isoeugenol + acetate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.318 EGS
-
 Ocimum basilicum
1.1.1.319 IGS
-
 Petunia x hybrida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.318 NADPH
-
 Ocimum basilicum
1.1.1.319 NADPH
-
 Petunia x hybrida