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Literature summary extracted from

  • Brooks, C.L.; Lazareno-Saez, C.; Lamoureux, J.S.; Mak, M.W.; Lemieux, M.J.
    Insights into substrate gating in H. influenzae rhomboid (2011), J. Mol. Biol., 407, 687-697.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.105 expressed in Escherichia coli Top10 cells Haemophilus influenzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.105
-
 Haemophilus influenzae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.105 F137A the mutant shows 14% of the wild type activity Haemophilus influenzae
3.4.21.105 F144A the mutation results in a 42% decrease in activity compared with the wild type enzyme Haemophilus influenzae
3.4.21.105 F160A the mutant decreases peptidase activity by 54% compared to wild type enzyme Haemophilus influenzae
3.4.21.105 F68A the substitution has no effect on activity (98% activity compared to the wild type enzyme) Haemophilus influenzae
3.4.21.105 F76A the mutation results in a 95% decrease in activity compared with the wild type enzyme Haemophilus influenzae
3.4.21.105 F84A the mutant shows 5% of the wild type activity Haemophilus influenzae
3.4.21.105 L136A the mutant shows 22% of the wild type activity Haemophilus influenzae
3.4.21.105 M164A the mutant decreases peptidase activity by 40% compared to wild type enzyme Haemophilus influenzae
3.4.21.105 W72A the mutant is not expressed and activity cannot be assessed Haemophilus influenzae
3.4.21.105 W72A/F76A/F144A the three alanine substitutions result in a 2.5fold increase in activity compared to wild type enzyme Haemophilus influenzae
3.4.21.105 W72V/F76V/F144V the three valine substitutions result in a 2fold increase in activity compared to wild type enzyme Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.105 Haemophilus influenzae P44783
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.105 Ni-NTA column chromatography and Superdex 200 gel filtration Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.105 C100Tat-Flag + H2O C100Tat-Flag is a chimera of the C-terminal 100 residues of APP, with seven residues of the Pseudomonas stuartii TatA cleavage site substituted at the N-terminus Haemophilus influenzae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.105 GlpG
-
 Haemophilus influenzae
3.4.21.105 Rhomboid
-
 Haemophilus influenzae